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Literature summary for 2.7.11.22 extracted from

  • Li, J.; Joo, S.H.; Tsai, M.D.
    An NF-kappaB-specific inhibitor, IkappaBalpha, binds to and inhibits cyclin-dependent kinase 4 (2003), Biochemistry, 42, 13476-13483.
    View publication on PubMed
Search for more literature for 2.7.11.22

Cloned(Commentary)

Cloned (Comment) Organism
expression of C-terminally His6-tagged wild-type and mutant CDK4 in insect cells using the baculovirus infection system with co-expression of cyclin D2, expression of CDK4 and inhibitor IkappaBalpha in the two-hybrid system in Saccharomyces cerevisiae Homo sapiens

Protein Variants

Protein Variants Comment Organism
K22Q site-directed mutagenesis, analysis of alterations in binding of INK4 proteins and IkappaBalpha compared to the wild-type enzyme Homo sapiens
N41S site-directed mutagenesis, analysis of alterations in binding of INK4 proteins and IkappaBalpha compared to the wild-type enzyme Homo sapiens
R24C site-directed mutagenesis, analysis of alterations in binding of INK4 proteins and IkappaBalpha compared to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
IkappaBalpha human protein, competes with INK4 proteins for binding sites on CDK4, CDK4 is bound at the N-terminal ankyrin repeats, while the C-terminal ankyrin repeats bind NF-kappaB, structure analysis Homo sapiens
additional information inhibition mechanism Homo sapiens
p16 wild-type and D84A mutant human INK4 protein, the mutant is only slightly inhibitory, p16 has regulatory function on the CDK4/cyclin D2 activity, p16 competes with IkappaBalpha for binding sites on CDK4, structure analysis Homo sapiens
p18 wild-type and D76A mutant human INK4 protein, the mutant is only slightly inhibitory, p18 competes with IkappaBalpha for binding sites on CDK4, structure analysis Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + retinoblastoma protein Homo sapiens i.e. Rb protein ADP + phosphorylated retinoblastoma protein
-
 ?
additional information Homo sapiens there is cross-talk between the NF-kappaB/IkappaBalpha pathway and the p16/CDK4/Rb pathway in cells with IkappaBalpha being capable to substitute for the inhibitory function of p16 on CDK4, regulation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged CDK4 from insect cells by affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + retinoblastoma protein i.e. Rb protein Homo sapiens ADP + phosphorylated retinoblastoma protein
-
 ?
additional information there is cross-talk between the NF-kappaB/IkappaBalpha pathway and the p16/CDK4/Rb pathway in cells with IkappaBalpha being capable to substitute for the inhibitory function of p16 on CDK4, regulation Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
CDK4
-
 Homo sapiens
cyclin-dependent kinase 4
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
cyclin D2 regulatory subunit of the holoenzyme formed with CDK4 Homo sapiens