Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + mitotic centromere-associated kinesin | Homo sapiens | i.e. MCAK, residue S621 in MCAK is the major phosphorylation site of Plk1, residues S632/S633 are also phoshorylated by Plk1. S632/S633 phosphorylation significantly enhances the microtubule depolymerizing activity of MCAK in vivo and in vitro | ADP + phosphorylated mitotic centromere-associated kinesin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NYY3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + mitotic centromere-associated kinesin | i.e. MCAK, residue S621 in MCAK is the major phosphorylation site of Plk1, residues S632/S633 are also phoshorylated by Plk1. S632/S633 phosphorylation significantly enhances the microtubule depolymerizing activity of MCAK in vivo and in vitro | Homo sapiens | ADP + phosphorylated mitotic centromere-associated kinesin | - |
? | |
ATP + mitotic centromere-associated kinesin | i.e. MCAK, residue S621 in MCAK is the major phosphorylation site of Plk1, residues S632/S633 are also phoshorylated by Plk1 | Homo sapiens | ADP + phosphorylated mitotic centromere-associated kinesin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the mitotic centromere-associated kinesin (MCAK), a potent microtubule depolymerase, is involved in regulating microtubule dynamics. The activity and subcellular localization of MCAK are tightly regulated by key mitotic kinases, such as Polo-like kinase 1 (Plk1) by phosphorylating multiple residues in MCAK. Plk1 phosphorylates very often different residues of substrates at different stages. Residue S621 in MCAK is the major phosphorylation site of Plk1, which is responsible for regulating MCAK's degradation by promoting the association of MCAK with APC/C_Cdc20. Plk1 also phosphorylates S632/S633 and regulates its catalytic activity in mitosis, this phosphorylation is required for proper spindle assembly during early phases of mitosis | Homo sapiens |