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Literature summary for 2.7.11.21 extracted from

  • Huang, Y.; Sun, L.; Liu, N.; Wei, Q.; Jiang, L.; Tong, X.; Ye, X.
    Polo-like kinase 1 (Plk1) up-regulates telomerase activity by affecting human telomerase reverse transcriptase (hTERT) stability (2015), J. Biol. Chem., 290, 18865-18873.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of FLAG-tagged wild-type Plk1, and FLAG-tagged mutants Plk1(1-330), Plk1(330-603), Plk1-TD (constitutively active form of Plk1), and Plk1-KD (kinase-deficient form of Plk1) in HEK293T cells, recombinant expression of His-tagged Plk1 in Escherichia coli strain BL21 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme knockdown in HeLa cells via transfection with Plk1-specific small interfering RNA (siRNA). Chromatin-bound hTERT is obviously decreased in Plk1 siRNA-transfected HeLa cells compared with that in control cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
chromatin
-
Homo sapiens 785
-
nucleus
-
Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens Plk1 can interact with hTERT independently of its kinase activity ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NYY3
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged Plk1 from Escherichia coli strain BL21, transfection of myc-Plk1, myc-Plk1-TD, or myc-Plk1-KD expression plasmids into H1299 cells leading to increased telomerase activity but without affecting the cell cycle progression in H1299 cells significantly Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
U2-OS cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Plk1 can interact with hTERT independently of its kinase activity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Plk1
-
Homo sapiens
Polo-like kinase 1
-
Homo sapiens

General Information

General Information Comment Organism
malfunction knockdown of Plk1 caused the reduction of telomerase activity, whereas overexpression of Plk1 increased telomerase activity. Overexpression of Plk1 leads to a significant increase of hTERT protein by prolonging its half-life but does not affect the level of hTERT mRNA Homo sapiens
physiological function hTERT plays a central role for telomerase activity and telomeric chromatin maintenance. Plk1 interacts with hTERT and regulates its stability. Overexpression of Plk1 up regulates the activity of telomerase. Plk1 enhances the chromatin loading of hTERT and inhibits its ubiquitination. Plk1 is a positive regulator of telomerase by enhancing the stability of hTERT, mechanism, overview. Plk1 can interact with hTERT and increase telomerase activity independently of its kinase activity Homo sapiens