Cloned (Comment) | Organism |
---|---|
recombinant expression of FLAG-tagged wild-type Plk1, and FLAG-tagged mutants Plk1(1-330), Plk1(330-603), Plk1-TD (constitutively active form of Plk1), and Plk1-KD (kinase-deficient form of Plk1) in HEK293T cells, recombinant expression of His-tagged Plk1 in Escherichia coli strain BL21 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knockdown in HeLa cells via transfection with Plk1-specific small interfering RNA (siRNA). Chromatin-bound hTERT is obviously decreased in Plk1 siRNA-transfected HeLa cells compared with that in control cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromatin | - |
Homo sapiens | 785 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | Plk1 can interact with hTERT independently of its kinase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NYY3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged Plk1 from Escherichia coli strain BL21, transfection of myc-Plk1, myc-Plk1-TD, or myc-Plk1-KD expression plasmids into H1299 cells leading to increased telomerase activity but without affecting the cell cycle progression in H1299 cells significantly | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
U2-OS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Plk1 can interact with hTERT independently of its kinase activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of Plk1 caused the reduction of telomerase activity, whereas overexpression of Plk1 increased telomerase activity. Overexpression of Plk1 leads to a significant increase of hTERT protein by prolonging its half-life but does not affect the level of hTERT mRNA | Homo sapiens |
physiological function | hTERT plays a central role for telomerase activity and telomeric chromatin maintenance. Plk1 interacts with hTERT and regulates its stability. Overexpression of Plk1 up regulates the activity of telomerase. Plk1 enhances the chromatin loading of hTERT and inhibits its ubiquitination. Plk1 is a positive regulator of telomerase by enhancing the stability of hTERT, mechanism, overview. Plk1 can interact with hTERT and increase telomerase activity independently of its kinase activity | Homo sapiens |