Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged Plk1 in Escherichia coli strain BL21-Gold(DE3) | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 53BP1 | Homo sapiens | 53BP1 is phosphorylated by Plk1 in mitosis | ADP + phosphorylated 53BP1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NYY3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
U2-OS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 53BP1 | 53BP1 is phosphorylated by Plk1 in mitosis | Homo sapiens | ADP + phosphorylated 53BP1 | - |
? | |
ATP + 53BP1 | 53BP1 is phosphorylated by Plk1 in its C-terminal domain at Ser1618 in the UDR domain | Homo sapiens | ADP + phosphorylated 53BP1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | 53BP1 is excluded from the chromatin in mitotic cells and did not colocalize with gH2AX in mitotic cells after damage through irradiation. Inhibition of Plk1 by BI2536 inhibitor arrests cells in mitosis and increases the mobility of 53BP. The removal of pS1618-53BP1 modification correlates to disappearance of pS10-histone H3 as well as degradation of cyclin B and Plk1 during mitotic exit | Homo sapiens |
physiological function | polo-like kinase 1 inhibits DNA damage response during mitosis, and Plk1 activity supresses DNA repair in mitotic cells. 53BP1 facilitates repair of DNA lesions through NHEJ especially when localized in the heterochromatin or at telomeres and is phosphorylated by Plk1 in mitosis, Upon phosphorylation of the N-terminal domain by ATM, 53BP1 recruits RIF1 and PTIP that block resection of DNA ends and promote repair through NHEJ. 53BP1 is recruited to the DNA damage foci through binding of its Tudor domain to the dimethylated histone H4K20-me2 and therefore 53BP1 is then recognized as a bivalent reader of posttranslationally modified mononucleosomes. Distinct behavior of 53BP1 in interphase and mitotic cells due to its phosphorylation. 53BP1 phosphorylation by Plk1 impairs its binding to ubiquitinated histones and localization to foci | Homo sapiens |