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Literature summary for 2.7.11.21 extracted from

  • Benada, J.; Burdova, K.; Lidak, T.; von Morgen, P.; Macurek, L.
    Polo-like kinase 1 inhibits DNA damage response during mitosis (2015), Cell Cycle, 14, 219-231.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged Plk1 in Escherichia coli strain BL21-Gold(DE3) Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 53BP1 Homo sapiens 53BP1 is phosphorylated by Plk1 in mitosis ADP + phosphorylated 53BP1
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NYY3
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-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
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U2-OS cell
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Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 53BP1 53BP1 is phosphorylated by Plk1 in mitosis Homo sapiens ADP + phosphorylated 53BP1
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?
ATP + 53BP1 53BP1 is phosphorylated by Plk1 in its C-terminal domain at Ser1618 in the UDR domain Homo sapiens ADP + phosphorylated 53BP1
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?

Synonyms

Synonyms Comment Organism
Plk1
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Homo sapiens
Polo-like kinase 1
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
malfunction 53BP1 is excluded from the chromatin in mitotic cells and did not colocalize with gH2AX in mitotic cells after damage through irradiation. Inhibition of Plk1 by BI2536 inhibitor arrests cells in mitosis and increases the mobility of 53BP. The removal of pS1618-53BP1 modification correlates to disappearance of pS10-histone H3 as well as degradation of cyclin B and Plk1 during mitotic exit Homo sapiens
physiological function polo-like kinase 1 inhibits DNA damage response during mitosis, and Plk1 activity supresses DNA repair in mitotic cells. 53BP1 facilitates repair of DNA lesions through NHEJ especially when localized in the heterochromatin or at telomeres and is phosphorylated by Plk1 in mitosis, Upon phosphorylation of the N-terminal domain by ATM, 53BP1 recruits RIF1 and PTIP that block resection of DNA ends and promote repair through NHEJ. 53BP1 is recruited to the DNA damage foci through binding of its Tudor domain to the dimethylated histone H4K20-me2 and therefore 53BP1 is then recognized as a bivalent reader of posttranslationally modified mononucleosomes. Distinct behavior of 53BP1 in interphase and mitotic cells due to its phosphorylation. 53BP1 phosphorylation by Plk1 impairs its binding to ubiquitinated histones and localization to foci Homo sapiens