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Literature summary for 2.7.11.21 extracted from

  • Park, J.E.; Li, L.; Park, J.; Knecht, R.; Strebhardt, K.; Yuspa, S.H.; Lee, K.S.
    Direct quantification of polo-like kinase 1 activity in cells and tissues using a highly sensitive and specific ELISA assay (2009), Proc. Natl. Acad. Sci. USA, 106, 1725-1730.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.21

Application

Application Comment Organism
analysis the development of an ELISA-based Plk1 assay is described that employs a principle to rapidly and accurately quantify the Plk1 activity with high sensitivity and specificity Mus musculus
analysis the development of an ELISA-based Plk1 assay is described that employs a principle to rapidly and accurately quantify the Plk1 activity with high sensitivity and specificity Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + a protein Mus musculus
-
 ADP + a phosphoprotein
-
 ?
ATP + a protein Homo sapiens
-
 ADP + a phosphoprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P53350
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
colon
-
 Mus musculus
-
heart
-
 Mus musculus
-
HeLa cell
-
 Homo sapiens
-
intestine
-
 Mus musculus
-
kidney
-
 Mus musculus
-
liver
-
 Mus musculus
-
lung
-
 Mus musculus
-
ovary
-
 Mus musculus
-
pancreas
-
 Mus musculus
-
spleen
-
 Mus musculus
-
stomach
-
 Mus musculus
-
testis
-
 Mus musculus
-
thymus
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + a protein
-
 Mus musculus ADP + a phosphoprotein
-
 ?
ATP + a protein
-
 Homo sapiens ADP + a phosphoprotein
-
 ?
ATP + centromeric protein PBIP1 Plk1 phosphorylates PBIP1 at T78, GST-PBIPtide is used as in vitro substrate Mus musculus ADP + phosphorylated centromeric protein PBIP1
-
 ?
ATP + centromeric protein PBIP1 Plk1 phosphorylates PBIP1 at T78, GST-PBIPtide is used as in vitro substrate Homo sapiens ADP + phosphorylated centromeric protein PBIP1
-
 ?

Synonyms

Synonyms Comment Organism
Plk1
-
 Mus musculus
Plk1
-
 Homo sapiens
Polo-like kinase 1
-
 Mus musculus
Polo-like kinase 1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 ELISA Mus musculus
30
-
 ELISA Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus
ATP
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens Plk is over-expressed in approximately 80% of human tumors of diverse origins up
Mus musculus Plk is over-expressed in tumors of diverse origins up