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Literature summary for 2.7.11.21 extracted from
- Interaction between human papillomavirus type 5 E2 and polo-like kinase 1 (2009), J. Med. Virol., 81, 536-544.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pBluescript II KS+, subsequently into pET15b+ for expression in Escherichia coli BL21DE3 cells, and into pEGFP-C1 | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTAKD | kinase domain deleted Plk1, residues 307-603 | Homo sapiens |
| DELTAPlk1 | polo box deleted Plk1, residues 1-400 | Homo sapiens |
| K82M | mutant | Homo sapiens |
| PBD | polo box domain, residues 401-603 | Homo sapiens |
| T210D | mutant | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| nucleus | speckled domains inside the nucleus | Homo sapiens | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | Homo sapiens | - |
ADP + a phosphoprotein | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P53350 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| hexahistidine-tagged Plk1 is purified using HIS-select nickel affinity agarose | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | skin epidermis | Homo sapiens | - |
| HeLa cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | - |
Homo sapiens | ADP + a phosphoprotein | - |
? | |
| ATP + Brd4 | the E2 binding domain of Brd4 is phosphorylated by Plk1 in vitro | Homo sapiens | ADP + phosphorylated Brd4 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Polo-like kinase 1 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | conserved function in mitotic processes and cytokinesis | Homo sapiens |
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Release 2023.1 (January 2023)
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