Cloned (Comment) | Organism |
---|---|
a DNA fragment encoding the N-terminus of the TbPLK gene product is cloned into the tetracycline-inducible RNAi vector pZJM for knockdown of TbPLK expression, for tagging proteins TbPLK is cloned into the pC-EYFP-Bla vector, a plasmid for expressing glutathione S-transferase-TbPLK is constructed by cloning a DNA fragment containing full-length TbPLK into the vector pET41a | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-(5,6-dimethoxy-1H-benzimidazole-1-yl)-3-[2-(trifluoromethyl)-benzyloxy]-thiophene-2-carboxamide | GW843682X | Trypanosoma brucei | |
RNAi | - |
Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | TbPLK trans-localizes from the flagellum attachment zone to the cytoplasm during anaphase | Trypanosoma brucei | 5737 | - |
flagellum | TbPLK trans-localizes from the flagellum attachment zone to the cytoplasm during anaphase | Trypanosoma brucei | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | Trypanosoma brucei | - |
ADP + a phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by glutathione-Sepharose 4B purification | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
Trypanosoma brucei | ADP + a phosphoprotein | - |
? | |
ATP + casein | activity assay | Trypanosoma brucei | ADP + a phosphorylated casein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
polo-like kinase | - |
Trypanosoma brucei |
TbPLK | - |
Trypanosoma brucei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
kinase assay | Trypanosoma brucei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
kinase assay | Trypanosoma brucei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trypanosoma brucei |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000022 | - |
- |
Trypanosoma brucei | 5-(5,6-dimethoxy-1H-benzimidazole-1-yl)-3-[2-(trifluoromethyl)-benzyloxy]-thiophene-2-carboxamide | |
0.0013 | - |
Escherichia coli-expressed recombinant GST-tagged TbPLK | Trypanosoma brucei | 5-(5,6-dimethoxy-1H-benzimidazole-1-yl)-3-[2-(trifluoromethyl)-benzyloxy]-thiophene-2-carboxamide |
General Information | Comment | Organism |
---|---|---|
physiological function | TbPLK regulates cytokinetic initiation | Trypanosoma brucei |