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Literature summary for 2.7.11.21 extracted from

  • Garcia-Alvarez, B.; de Carcer, G.; Ibanez, S.; Bragado-Nilsson, E.; Montoya, G.
    Molecular and structural basis of polo-like kinase 1 substrate recognition: Implications in centrosomal localization (2007), Proc. Natl. Acad. Sci. USA, 104, 3107-3112.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information substrate binding to Plk1 is supposed to promote its activity Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
Plk1 cloned into vector pFastBac, the polo box domain cDNA sequence (residues 367-603) cloned into vector pGEX-6P-2. PC3 cells, HeLa cells and NIH 3T3 cells transfected with either EGFP-Plk1 or EGFP-Plk1 H538A/K540M Homo sapiens
the Plk cDNA sequence is cloned into a pFastBac vector to generate a recombinant baculovirus, the polo box domain cDNA sequence, residues 367-603, is cloned into vector pGEX-6P-2 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of the PBD in complex with nonphysiological and control target peptides Cdc25C and Cdc25C-P, to 1.95 A, 2.10 A, and 2.80 A resolution, Trp-414 is fundamental in substrate recognition regardless of its phosphorylation status Homo sapiens
the crystal structures of the polo box domain and its complexes with Cdc25C-P and Cdc25C peptides are solved by nuclear replacement and refined to 1.95, 2.10, and 2.80 A resolution Homo sapiens

Protein Variants

Protein Variants Comment Organism
H538A/K540M localizes at the centrosome, when wild-type Plk1 and H538A/K540M are fused to EGFP. After photobleaching recovers its signal at the centrosome following very similar kinetics as the wild-type Homo sapiens
H538A/K540M double mutant, no differences in centrosomal localization compared to the wild-type protein are detected Homo sapiens
additional information EGFP-Plk1 delta400-603 mutant, lacks the polo box domain, localizes to the centrosome and displays a similar fluorescence intensity to the W414F mutant Homo sapiens
W414F abolishes molecular recognition and diminishes centrosomal localization Homo sapiens
W414F mutation abolishes polo box domain target peptide binding and reduces EGFP-Plk1 centrosomal localization Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
centrosome
-
Homo sapiens 5813
-
centrosome after transfection into PC3 cells, HeLa cells and NIH 3T3 cells, Plk1 localization is controlled not only by the polo box domain, the kinase domain is also involved in Plk1 targeting mechanism to the centrosome Homo sapiens 5813
-
spindle
-
Homo sapiens 5819
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + protein Homo sapiens
-
ADP + phosphoprotein
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens P53350
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
by Ni2+ chromatography and gel-filtration Homo sapiens
the protein is purified in a Ni2+ column and by gel filtration chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
PC-3 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Cdc25C the polo box domain displays a similar affinity for non-phosphorylated and phosphorylated Cdc25C target peptides, full-length Plk1 shows ca. 7fold more affinity for the phosphorylated peptide Homo sapiens ADP + phosphorylated Cdc25C
-
?
ATP + Cdc25C
-
Homo sapiens ADP + phosphorylated Cdc25
-
?
ATP + cyclin B
-
Homo sapiens ADP + phosphorylated cyclin B
-
?
ATP + histone H1 substrate used in kinase assay Homo sapiens ADP + phosphorylated histone H1
-
?
ATP + Myt1
-
Homo sapiens ADP + phosphorylated Myt1
-
?
ATP + protein
-
Homo sapiens ADP + phosphoprotein
-
?
ATP + SCC1 cohesin
-
Homo sapiens ADP + phosphorylated SCC1 cohesin
-
?

Synonyms

Synonyms Comment Organism
Plk1
-
Homo sapiens
Polo-like kinase 1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
kinase assay Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens