Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | substrate binding to Plk1 is supposed to promote its activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
Plk1 cloned into vector pFastBac, the polo box domain cDNA sequence (residues 367-603) cloned into vector pGEX-6P-2. PC3 cells, HeLa cells and NIH 3T3 cells transfected with either EGFP-Plk1 or EGFP-Plk1 H538A/K540M | Homo sapiens |
the Plk cDNA sequence is cloned into a pFastBac vector to generate a recombinant baculovirus, the polo box domain cDNA sequence, residues 367-603, is cloned into vector pGEX-6P-2 | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structures of the PBD in complex with nonphysiological and control target peptides Cdc25C and Cdc25C-P, to 1.95 A, 2.10 A, and 2.80 A resolution, Trp-414 is fundamental in substrate recognition regardless of its phosphorylation status | Homo sapiens |
the crystal structures of the polo box domain and its complexes with Cdc25C-P and Cdc25C peptides are solved by nuclear replacement and refined to 1.95, 2.10, and 2.80 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
H538A/K540M | localizes at the centrosome, when wild-type Plk1 and H538A/K540M are fused to EGFP. After photobleaching recovers its signal at the centrosome following very similar kinetics as the wild-type | Homo sapiens |
H538A/K540M | double mutant, no differences in centrosomal localization compared to the wild-type protein are detected | Homo sapiens |
additional information | EGFP-Plk1 delta400-603 mutant, lacks the polo box domain, localizes to the centrosome and displays a similar fluorescence intensity to the W414F mutant | Homo sapiens |
W414F | abolishes molecular recognition and diminishes centrosomal localization | Homo sapiens |
W414F | mutation abolishes polo box domain target peptide binding and reduces EGFP-Plk1 centrosomal localization | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
centrosome | - |
Homo sapiens | 5813 | - |
centrosome | after transfection into PC3 cells, HeLa cells and NIH 3T3 cells, Plk1 localization is controlled not only by the polo box domain, the kinase domain is also involved in Plk1 targeting mechanism to the centrosome | Homo sapiens | 5813 | - |
spindle | - |
Homo sapiens | 5819 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protein | Homo sapiens | - |
ADP + phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | P53350 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
by Ni2+ chromatography and gel-filtration | Homo sapiens |
the protein is purified in a Ni2+ column and by gel filtration chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
PC-3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Cdc25C | the polo box domain displays a similar affinity for non-phosphorylated and phosphorylated Cdc25C target peptides, full-length Plk1 shows ca. 7fold more affinity for the phosphorylated peptide | Homo sapiens | ADP + phosphorylated Cdc25C | - |
? | |
ATP + Cdc25C | - |
Homo sapiens | ADP + phosphorylated Cdc25 | - |
? | |
ATP + cyclin B | - |
Homo sapiens | ADP + phosphorylated cyclin B | - |
? | |
ATP + histone H1 | substrate used in kinase assay | Homo sapiens | ADP + phosphorylated histone H1 | - |
? | |
ATP + Myt1 | - |
Homo sapiens | ADP + phosphorylated Myt1 | - |
? | |
ATP + protein | - |
Homo sapiens | ADP + phosphoprotein | - |
? | |
ATP + SCC1 cohesin | - |
Homo sapiens | ADP + phosphorylated SCC1 cohesin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Plk1 | - |
Homo sapiens |
Polo-like kinase 1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
kinase assay | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |