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Literature summary for 2.7.11.21 extracted from

  • Zhang, W.; Fletcher, L.; Muschel, R.J.
    The role of polo-like kinase 1 in the inhibition of centrosome separation after ionizing radiation (2005), J. Biol. Chem., 280, 42994-42999.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in U2OS cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information Plk1 depletion by siRNA transfection combined with Nek2 overexpression in U2OS cells Homo sapiens
T210D site-directed mutagenesis, constitutively active mutant, overrides the irradiation-induced DNA damage and inhibition of centrosome separation in contrast to the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information in vivo inhibition of Plk1 by DNA damage is ATM- or ATR-dependent Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
Homo sapiens
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is involved in regulation of Nek2-induced centrosome separation after DNA damage ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
Homo sapiens
-
U2-OS cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein
-
Homo sapiens ADP + phosphorylated casein
-
?
additional information the enzyme is involved in regulation of Nek2-induced centrosome separation after DNA damage Homo sapiens ?
-
?
additional information the protein phosphatase 1 is no substrate of Plk1 Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Plk1
-
Homo sapiens
Polo-like kinase 1
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens