Literature summary for 2.7.11.21 extracted from

Eckerdt, F.; Yuan, J.; Saxena, K.; Martin, B.; Kappel, S.; Lindenau, C.; Kramer, A.; Naumann, S.; Daum, S.; Fischer, G.; Dikic, I.; Kaufmann, M.; Strebhardt, K.
Polo-like kinase 1-mediated phosphorylation stabilizes Pin1 by inhibiting its ubiquitination in human cells (2005), J. Biol. Chem., 280, 36575-36583.

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Cloned(Commentary)

Cloned (Comment)	Organism
stable expression of Plk1 and transfection with hairpin shRNA in HeLa cells, expression of GST-tagged Plk1 in Spodoptera frugiperda Sf9 cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K82M	site-directed mutagenesis, kinase-defective Plk1 mutant	Homo sapiens
additional information	down-regulation of Plk1 by expression of hairpin shRNA results in increased Pin1 ubiquitination and to down-regulation of pin 1 levels during mitosis	Homo sapiens
T210D	site-directed mutagenesis, hyperactive Plk1 mutant, shows reduced activity with truncated Pin1 compared to the wild-tpe Plk1	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + Pin1	Homo sapiens	Plk1-mediated phosphorylation at Ser65 stabilizes Pin1 by inhibiting its ubiquitination in cells	ADP + phosphorylated Pin1	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P53350	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged Plk1 from Spodoptera frugiperda Sf9 cells	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293T cell	-	Homo sapiens	-
HeLa-S3 cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + Pin1	Plk1-mediated phosphorylation at Ser65 stabilizes Pin1 by inhibiting its ubiquitination in cells	Homo sapiens	ADP + phosphorylated Pin1	-	?
ATP + Pin1	recombinant isomerase Pin1 expressed in Escherichia coli, phosphorylation at Ser65, wild-type and truncated mutant Pin1 comprising residues 1-70	Homo sapiens	ADP + phosphorylated Pin1	-	?
ATP + Plk1 polo box 1-derived peptide	synthetic substrate	Homo sapiens	ADP + phosphorylated Plk1 polo box 1-derived peptide	-	?

Synonyms

Synonyms	Comment	Organism
Plk1	-	Homo sapiens
Polo-like kinase 1	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

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Release 2023.1 (January 2023)