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Literature summary for 2.7.11.21 extracted from
- POLO kinase regulates the Drosophila centromere cohesion protein MEI-S332 (2005), Dev. Cell, 8, 53-64.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-tagged Plx1 in S2 cells, co-expression with GFP-tagged MEI-S332 | Drosophila melanogaster |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | male homozygous polo mutant flies die in the third instar larval stage, heterozygous mutants show affected centromer dissociation of MEI-S332, but not association resulting in chromosome segragation defects | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MEI-S332 protein | Xenopus laevis | phosphorylation/polo box binding is required for chromosomal dissociation of MEI-S332 | ADP + phosphorylated MEI-S332 protein | - |
? |  |
| ATP + MEI-S332 protein | Drosophila melanogaster | substrate is a protein essential in meiosis for maintaining cohesion at centromers until sister chromatids separate at the metaphase II/anaphase II transition, phosphorylation by polo kinase removes the protein from centromeres and antagonizes the MEI-S332 function, MEI-S332 phosphorylation by polo kinase is essential for viability of the cells | ADP + phosphorylated MEI-S332 protein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | - |
gene polo | - |
| Xenopus laevis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Xenopus laevis | - |
| SCHNEIDER-2 cell | embryonic cell line | Drosophila melanogaster | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + MEI-S332 protein | phosphorylation/polo box binding is required for chromosomal dissociation of MEI-S332 | Xenopus laevis | ADP + phosphorylated MEI-S332 protein | - |
? | |
| ATP + MEI-S332 protein | substrate is a protein essential in meiosis for maintaining cohesion at centromers until sister chromatids separate at the metaphase II/anaphase II transition, phosphorylation by polo kinase removes the protein from centromeres and antagonizes the MEI-S332 function, MEI-S332 phosphorylation by polo kinase is essential for viability of the cells | Drosophila melanogaster | ADP + phosphorylated MEI-S332 protein | - |
? | |
| ATP + MEI-S332 protein | enzyme requires residues T331 and possibly in combination with S234 for activity, no activity with MEI-S332 protein mutants T331A and S234A/T331A | Xenopus laevis | ADP + phosphorylated MEI-S332 protein | - |
? | |
| ATP + MEI-S332 protein | enzyme requires residues T331 and possibly in combination with S234 for activity, no activity with MEI-S332 protein mutants T331A and S234A/T331A in S2 cells | Drosophila melanogaster | ADP + phosphorylated MEI-S332 protein | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Plx1 | - |
Xenopus laevis |
| Polo kinase | - |
Drosophila melanogaster |
| Polo-like kinase 1 | - |
Xenopus laevis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Drosophila melanogaster | |
| ATP | - |
Xenopus laevis | |
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