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Literature summary for 2.7.11.21 extracted from
- Mad3/BubR1 phosphorylation during spindle checkpoint activation depends on both Polo and Aurora kinases in budding yeast (2005), Cell Cycle, 4, 972-980.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of CDC5 fused to the Met3 promoter intergrated in the LEU2 locus of a yeast strain carrying a null allele of CDC5 | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| kinetochore | - |
Saccharomyces cerevisiae | 776 | - |
| spindle | - |
Saccharomyces cerevisiae | 5819 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Saccharomyces cerevisiae |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Mad3 | Saccharomyces cerevisiae | phosphorylation of Mad3 isozymes at 5 serine residues, S222, S380, S466, S504, and especially at S268, during spindle checkpoint activation, Mad3 is an inhibitor of Cdc20/APC ubiquitin ligase, overview | ADP + phosphorylated Mad3 | - |
? | |
| additional information | Saccharomyces cerevisiae | Plk1 is important in activation and regulation of spindle assembly during mitosis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
diverse strains derived from strain W303 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Mad3 | phosphorylation of Mad3 isozymes at 5 serine residues, S222, S380, S466, S504, and especially at S268, during spindle checkpoint activation, Mad3 is an inhibitor of Cdc20/APC ubiquitin ligase, overview | Saccharomyces cerevisiae | ADP + phosphorylated Mad3 | - |
? | |
| ATP + Mad3 | recombinant HA-tagged wild-type and mutant Mad3 substrate proteins, the activity with mutants in which phorylation site Ser is replaced by Ala is highly reduced, Mad3 is a homologue to the human BubR1 protein | Saccharomyces cerevisiae | ADP + phosphorylated Mad3 | - |
? | |
| additional information | Plk1 is important in activation and regulation of spindle assembly during mitosis | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cdc5 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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