Cloned (Comment) | Organism |
---|---|
expression of CDC5 fused to the Met3 promoter intergrated in the LEU2 locus of a yeast strain carrying a null allele of CDC5 | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
kinetochore | - |
Saccharomyces cerevisiae | 776 | - |
spindle | - |
Saccharomyces cerevisiae | 5819 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Mad3 | Saccharomyces cerevisiae | phosphorylation of Mad3 isozymes at 5 serine residues, S222, S380, S466, S504, and especially at S268, during spindle checkpoint activation, Mad3 is an inhibitor of Cdc20/APC ubiquitin ligase, overview | ADP + phosphorylated Mad3 | - |
? | |
additional information | Saccharomyces cerevisiae | Plk1 is important in activation and regulation of spindle assembly during mitosis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
diverse strains derived from strain W303 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Mad3 | phosphorylation of Mad3 isozymes at 5 serine residues, S222, S380, S466, S504, and especially at S268, during spindle checkpoint activation, Mad3 is an inhibitor of Cdc20/APC ubiquitin ligase, overview | Saccharomyces cerevisiae | ADP + phosphorylated Mad3 | - |
? | |
ATP + Mad3 | recombinant HA-tagged wild-type and mutant Mad3 substrate proteins, the activity with mutants in which phorylation site Ser is replaced by Ala is highly reduced, Mad3 is a homologue to the human BubR1 protein | Saccharomyces cerevisiae | ADP + phosphorylated Mad3 | - |
? | |
additional information | Plk1 is important in activation and regulation of spindle assembly during mitosis | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cdc5 | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Saccharomyces cerevisiae |