Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L2 domain of pyruvate dehydrogenase complex | binding to the inner lipoyl domains L2 of E2 component activate the enzyme by conformational changes and disrupting the ATP lid and eliminating product inhibition by ADP, binding structure, overview, L2 binding increases affinities for both ADP and ATP | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
pyruvate dehydrogenase kinase 3 bound to ATP and the lipoyl domain 2 of human pyruvate dehydrogenase complex, X-ray structure determination and analysis at 1.0 A resolution, modeling of closed and open conformation | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | product inhibition, L2 binding increases affinities for both ADP and ATP | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dissociation constants for ADP and ATP binding, L2 binding increases affinities for both ADP and ATP | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate dehydrogenase | Homo sapiens | - |
ADP + phosphorylated pyruvate dehydrogenase | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)]phosphate | allosteric R-T equilibrium as the mechanism for L2-stimulated PDK3 activity, the active cleft undergoes a conformational change from closed to open conformation upon stimulation by L2 binding releasing ADP | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate dehydrogenase | - |
Homo sapiens | ADP + phosphorylated pyruvate dehydrogenase | - |
ir | |
ATP + pyruvate dehydrogenase | phosphorylation of serine residues of the E1 component of pyruvate dehydrogenase complex PDC | Homo sapiens | ADP + phosphorylated pyruvate dehydrogenase | - |
ir |
Subunits | Comment | Organism |
---|---|---|
dimer | PDK is a component of the pyruvate dehydrogenase complex bound to the inner lipoyl domains, i.e. L2, of the E2 component, the C-terminus of one PDK subunit constitutes an integral part of the lipoyl-binding pocket in the N-terminus of the opposing other subunit | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PDK3 | - |
Homo sapiens |
pyruvate dehydrogenase kinase 3 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | L2 binding increases affinities for both ADP and ATP | Homo sapiens | |
ATPgammaS | dissociation constants as ATP in binding to PDK3 | Homo sapiens |