Activating Compound | Comment | Organism | Structure |
---|---|---|---|
inner lipoyl-bearing domain 2 | i.e. L2, binding to the inner lipoyl domain L2 of E2 component of the PDC enzyme complex activates and regulates the enzyme by conformational changes and disrupting the ATP lid and eliminating product inhibition by ADP, mechanism, L2 binding strongly decreases PDHK2 affinity for ATP, strongest activation occurrs in presence of E1, E1-binding domain, and E2, overview | Homo sapiens | |
additional information | effects of recombinant E2 component-derived deletion constructs on PDK activity, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
co-expression of GST-tagged PDHK2 with E1 and E2 components of PDC in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several E2 component-derived domains, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | effects of recombinant E2 component-derived deletion constructs on PDK activity, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate dehydrogenase | Homo sapiens | - |
ADP + phosphorylated pyruvate dehydrogenase | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + pyruvate dehydrogenase | phosphorylation of serine residues of the E1 PDC component | Homo sapiens | ADP + phosphorylated pyruvate dehydrogenase | - |
ir | |
ATP + pyruvate dehydrogenase | - |
Homo sapiens | ADP + phosphorylated pyruvate dehydrogenase | - |
ir | |
ATP + pyruvate dehydrogenase | phosphorylation of serine residues of the E1 PDC component | Homo sapiens | ADP + phosphorylated pyruvate dehydrogenase | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | PDK is a component of the pyruvate dehydrogenase complex, i.e. PDC, the E2 component of PDC comprises the transacetylase domain, the E1-binding domain, and two lipoyl-bearing domains L1 and L2 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PDHK2 | - |
Homo sapiens |
pyruvate dehydrogenase kinase 2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | causes a decrease in PDHK2 affinity for the L2 domain | Homo sapiens | |
additional information | no activity with GTP | Homo sapiens |