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Literature summary for 2.7.11.2 extracted from

  • Tuganova, A.; Popov, K.M.
    Role of protein-protein interactions in the regulation of pyruvate dehydrogenase kinase activity (2005), Biochem. J., 387, 147-153.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
inner lipoyl-bearing domain 2 i.e. L2, binding to the inner lipoyl domain L2 of E2 component of the PDC enzyme complex activates and regulates the enzyme by conformational changes and disrupting the ATP lid and eliminating product inhibition by ADP, mechanism, L2 binding strongly decreases PDHK2 affinity for ATP, strongest activation occurrs in presence of E1, E1-binding domain, and E2, overview Homo sapiens
additional information effects of recombinant E2 component-derived deletion constructs on PDK activity, overview Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
co-expression of GST-tagged PDHK2 with E1 and E2 components of PDC in Escherichia coli strain BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of several E2 component-derived domains, overview Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information effects of recombinant E2 component-derived deletion constructs on PDK activity, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pyruvate dehydrogenase Homo sapiens
-
ADP + phosphorylated pyruvate dehydrogenase
-
ir

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + pyruvate dehydrogenase phosphorylation of serine residues of the E1 PDC component Homo sapiens ADP + phosphorylated pyruvate dehydrogenase
-
ir
ATP + pyruvate dehydrogenase
-
Homo sapiens ADP + phosphorylated pyruvate dehydrogenase
-
ir
ATP + pyruvate dehydrogenase phosphorylation of serine residues of the E1 PDC component Homo sapiens ADP + phosphorylated pyruvate dehydrogenase
-
ir

Subunits

Subunits Comment Organism
More PDK is a component of the pyruvate dehydrogenase complex, i.e. PDC, the E2 component of PDC comprises the transacetylase domain, the E1-binding domain, and two lipoyl-bearing domains L1 and L2 Homo sapiens

Synonyms

Synonyms Comment Organism
PDHK2
-
Homo sapiens
pyruvate dehydrogenase kinase 2
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP causes a decrease in PDHK2 affinity for the L2 domain Homo sapiens
additional information no activity with GTP Homo sapiens