Any feedback?
Literature summary for 2.7.11.2 extracted from
- Analysis of the catalytic mechanism of pyruvate dehydrogenase kinase (2005), Arch. Biochem. Biophys., 434, 159-168.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PDK DNA sequence determination and analysis, expression of His6-tagged wild-type and mutant PDKs in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E238A | site-directed mutagenesis, highly reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
| E238H | site-directed mutagenesis, highly reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
| E238Q | site-directed mutagenesis, highly reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
| H233A | site-directed mutagenesis, reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
| K241A | site-directed mutagenesis, highly reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
| L234A | site-directed mutagenesis, reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
| L234H | site-directed mutagenesis, reduced activity compared to the wild-type PDK | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Arabidopsis thaliana | |
| 0.05 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant L234H | Arabidopsis thaliana | |
| 0.05 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant wild-type PDK | Arabidopsis thaliana | |
| 0.075 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant L234A | Arabidopsis thaliana | |
| 0.2 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant H233A | Arabidopsis thaliana | |
| 0.28 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant E238A | Arabidopsis thaliana | |
| 0.4 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant E238Q | Arabidopsis thaliana | |
| 3.5 | - |
pyruvate dehydrogenase | about, pH 7.5, 37°C, recombinant PDK mutant E238H | Arabidopsis thaliana |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Arabidopsis thaliana |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate dehydrogenase | Arabidopsis thaliana | - |
ADP + phosphorylated pyruvate dehydrogenase | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9SBJ1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged wild-type and mutant PDKs from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate dehydrogenase | - |
Arabidopsis thaliana | ADP + phosphorylated pyruvate dehydrogenase | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDK | - |
Arabidopsis thaliana |
| pyruvate dehydrogenase kinase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at, temperature-dependency of wild-type and mutant PDKs, overview | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0004 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant E238A | Arabidopsis thaliana | |
| 0.0005 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant E238H | Arabidopsis thaliana | |
| 0.07 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant L234A | Arabidopsis thaliana | |
| 0.28 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant H233A | Arabidopsis thaliana | |
| 0.39 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant PDK mutant L234H | Arabidopsis thaliana | |
| 0.55 | - |
pyruvate dehydrogenase | pH 7.5, 37°C, recombinant wild-type PDK | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at, pH-dependency of wild-type and mutant PDKs, overview | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
