Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination, functional expression as soluble protein in Escherichia coli strain HMS 174 (DE3) | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dichloroacetate | highly specific | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
x * 36000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
48000 | - |
x * 48000, native enzyme, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Rattus norvegicus | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q63065 | from heart | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli, large scale | Rattus norvegicus |
Renatured (Comment) | Organism |
---|---|
reconstitution after solubilization with 6 M urea | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Rattus norvegicus | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, native enzyme, SDS-PAGE | Rattus norvegicus |
? | x * 36000, recombinant enzyme, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | PDK isozymes and the related branched-chain dehydrogenase kinase form a unique family of serine kinases | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Rattus norvegicus |