Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | kinase bound to transacetylase core | Caenorhabditis elegans | |
acetyl-CoA | kinase bound to transacetylase core | Ascaris suum | |
acetyl-CoA | synergism with NADH | Caenorhabditis elegans | |
acetyl-CoA | synergism with NADH | Ascaris suum | |
dihydrolipoyl transacetylase | pyruvate dehydrogenase-complex transacetylase core | Caenorhabditis elegans | |
dihydrolipoyl transacetylase | pyruvate dehydrogenase-complex transacetylase core | Ascaris suum | |
NADH | kinase bound to transacetylase core | Caenorhabditis elegans | |
NADH | kinase bound to transacetylase core | Ascaris suum | |
NADH | synergism with acetyl-CoA | Caenorhabditis elegans | |
NADH | synergism with acetyl-CoA | Ascaris suum |
Cloned (Comment) | Organism |
---|---|
expression of truncated enzyme forms, comprising residues 284-402 and 1-334, respectively, as maltose-binding-protein fusion proteins in Escherichia coli JM109 | Caenorhabditis elegans |
functional expression in Escherichia coli as His-tagged protein | Caenorhabditis elegans |
functional expression in Escherichia coli strain BL21(DE3) as His-tagged protein | Ascaris suum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of truncated enzyme forms, the N-terminally truncated form, residues 284-402, is catalytically inactive, the C-terminally reduced form, residues 1-334, shows reduced activity | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Trypsin | - |
Ascaris suum | |
Trypsin | - |
Caenorhabditis elegans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Caenorhabditis elegans | |
Mg2+ | - |
Ascaris suum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, recombinant wild-type, SDS-PAGE | Caenorhabditis elegans |
76000 | - |
recombinant wild-type, gel filtration | Caenorhabditis elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Caenorhabditis elegans | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | Ascaris suum | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | O02623 | - |
- |
Caenorhabditis elegans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli as His-tagged protein to homogeneity | Ascaris suum |
recombinant wild-type and truncated form from Escherichia coli as His-tagged and maltose-binding fusion protein, respectively, to homogeneity | Caenorhabditis elegans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Ascaris suum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.74 | - |
purified recombinant enzyme, in presence of NADH and acetyl-CoA | Caenorhabditis elegans |
0.92 | - |
purified recombinant enzyme, in presence of NADH and acetyl-CoA | Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Caenorhabditis elegans | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Ascaris suum | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Caenorhabditis elegans | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Ascaris suum | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
additional information | no autophosphorylation | Caenorhabditis elegans | ? | - |
? | |
additional information | no autophosphorylation | Ascaris suum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Ascaris suum |
dimer | 2 * 45000, recombinant wild-type, SDS-PAGE | Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Caenorhabditis elegans |
37 | - |
assay at | Ascaris suum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Caenorhabditis elegans |
7.4 | - |
assay at | Ascaris suum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Caenorhabditis elegans | |
ATP | dependent on | Ascaris suum |