Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | kinase bound to transacetylase core | Ascaris suum | |
acetyl-CoA | synergism with NADH | Ascaris suum | |
dihydrolipoyl transacetylase | pyruvate dehydrogenase-complex transacetylase core | Ascaris suum | |
NADH | kinase bound to transacetylase core | Ascaris suum | |
NADH | synergism with acetyl-CoA | Ascaris suum |
Cloned (Comment) | Organism |
---|---|
DNA sequence determination and analysis | Ascaris suum |
functional expression in Escherichia coli strain BL21(DE3) as His-tagged protein | Ascaris suum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | enzyme contains a 18-amino acid mitochondrial import signal sequence | Ascaris suum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Ascaris suum | |
Mg2+ | actual substrate: MgATP2- | Ascaris suum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
about, recombinant enzyme, gel filtration | Ascaris suum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | Ascaris suum | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ascaris suum | O02623 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli as His-tagged protein to homogeneity | Ascaris suum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | adult | Ascaris suum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Ascaris suum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [pyruvate dehydrogenase (lipoamide)] | - |
Ascaris suum | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
ATP + [pyruvate dehydrogenase (lipoamide)] | catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 | Ascaris suum | ADP + [pyruvate dehydrogenase (lipoamide)] phosphate | - |
ir | |
additional information | no autophosphorylation | Ascaris suum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000-43470, mature protein, SDS-PAGE and DNA sequence determination | Ascaris suum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ascaris suum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Ascaris suum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Ascaris suum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Ascaris suum | mature protein | - |
5.9 |