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Literature summary for 2.7.11.2 extracted from

  • Stepp, L.R.; Pettit, F.H.; Yeaman, S.J.; Reed, L.J.
    Purification and properties of pyruvate dehydrogenase kinase from bovine kidney (1983), J. Biol. Chem., 258, 9454-9458.
    View publication on PubMed
Search for more literature for 2.7.11.2

Activating Compound

Activating Compound Comment Organism Structure
dihydrolipoyl transacetylase
-
 Bos taurus

General Stability

General Stability Organism
labile to freeze-thawing Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
chymotrypsin proteolysis of kinase alpha-, not beta-subunit, no inactivation by trypsin-mediated proteolysis of beta-subunit Bos taurus
additional information no inhibition by cAMP; no inhibition by cGMP; not affected by calmodulin with or without Ca2+ Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
48000
-
 1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus beta-subunit harbors a regulatory role ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2700fold to homogeneity Bos taurus
kidney, from highly purifed pyruvate dehydrogenase-complex Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.33
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
4°C, 0.02% NaN3, t1/2: 1 month Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [pyruvate dehydrogenase (lipoamide)] highly specific for the substrate Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
ATP + [pyruvate dehydrogenase (lipoamide)] beta-subunit harbors a regulatory role Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
additional information little, if any activity with casein of bovine kidney Bos taurus ?
-
 ?
additional information no activity with histones of calf thymus type II-A, VI-S and VIII-S Bos taurus ?
-
 ?
additional information no activity with glycogen synthase a and rabbit skeletal muscle phosphorylase b Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
More kinase activity resides in alpha-subunit Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Bos taurus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Bos taurus
additional information no activation by cAMP Bos taurus
additional information no activation by Ca2+/calmodulin or calmodulin alone Bos taurus
additional information no activation by cGMP Bos taurus