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Literature summary for 2.7.11.19 extracted from

  • Hayes, J.; Skamnaki, V.; Archontis, G.; Lamprakis, C.; Sarrou, J.; Bischler, N.; Skaltsounis, A.; Zographos, S.; Oikonomakos, N.
    Kinetics, in silico docking, molecular dynamics, and MM-GBSA binding studies on prototype indirubins, KT5720, and staurosporine as phosphorylase kinase ATP-binding site inhibitors: The role of water molecules examined (2011), Proteins, 79, 703-719.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed as a N-terminal GST fusion, expressed in B834 (DE3)pLyS cells at 18°C Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
indirubin phosphorylase kinase ATP-binding site inhibitor Oryctolagus cuniculus
indirubin-3'-oxime phosphorylase kinase ATP-binding site inhibitor Oryctolagus cuniculus
KT5720 phosphorylase kinase ATP-binding site inhibitor Oryctolagus cuniculus
staurosporine phosphorylase kinase ATP-binding site inhibitor Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + glycogen phosphorylase b
-
 Oryctolagus cuniculus 2 ADP + glycogen phosphorylase a
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
 assay at Oryctolagus cuniculus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000037
-
 KT5720 30°C, pH 8.2 Oryctolagus cuniculus
0.0000184
-
 staurosporine 30°C, pH 8.2 Oryctolagus cuniculus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00000037
-
 30°C, pH 8.2 Oryctolagus cuniculus staurosporine
0.0000184
-
 30°C, pH 8.2 Oryctolagus cuniculus KT5720
0.000144
-
 30°C, pH 8.2 Oryctolagus cuniculus indirubin-3'-oxime
0.05
-
 30°C, pH 8.2 Oryctolagus cuniculus indirubin