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Literature summary for 2.7.11.19 extracted from

  • Lanciotti, R.A.; Bender, P.K.
    Baculovirus-directed expression of the gamma-subunit of phosphorylase kinase: purification and calmodulin dependence (1994), Biochem. J., 299, 183-189.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
mouse catalytic gamma subunit, Baculovirus-directed expression in Sf9 insect cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 phosphorylase b recombinant gamma subunit, pH 6.8 Mus musculus
0.03 0.037 ATP phosphorylase b Mus musculus
0.03 0.037 ATP recombinant gamma subunit, pH 8.2 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Mus musculus
Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Mus musculus
phosphate requirement, phosphate containing enzyme Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant gamma-subunit, as expressed in Sf-9 cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + phosphorylase b cosubstrate: Mg-ATP complex Mus musculus ADP + phosphorylase a
-
 ?
ATP + phosphorylase b rabbit skeletal muscle Mus musculus ADP + phosphorylase a
-
 ?

Cofactor

Cofactor Comment Organism Structure
Calmodulin additional activation Mus musculus
Calmodulin activation of recombinant gamma subunit at pH 6.8, slightly at pH 8.2 Mus musculus