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Literature summary for 2.7.11.19 extracted from
- Characterization of phosphorylase kinase activities in yeast (1983), Biochem. Biophys. Res. Commun., 114, 331-338.
General Stability
| General Stability | Organism |
|---|---|
| 5%, w/v, glycerol stabilizes | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | not | Saccharomyces cerevisiae |  |
| additional information | no inhibition by cAMP-binding protein | Saccharomyces cerevisiae |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
ATP | - |
Saccharomyces cerevisiae | |
| 0.6 | - |
GTP | - |
Saccharomyces cerevisiae | |
| 1.4 | - |
UTP | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
| soluble | - |
Saccharomyces cerevisiae | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Saccharomyces cerevisiae | |
| Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Saccharomyces cerevisiae | |
| phosphate | requirement, phosphate containing enzyme | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
commercial baker's yeast | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + glycogen synthase | at high concentration, from yeast | Saccharomyces cerevisiae | ADP + phosphoglycogen synthase | - |
? | |
| ATP + phosphorylase b | yeast | Saccharomyces cerevisiae | ADP + phosphorylase a | - |
? | |
| ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Saccharomyces cerevisiae | ADP + phosphorylase a | - |
? | |
| ATP + phosphorylase b | rabbit skeletal muscle | Saccharomyces cerevisiae | ADP + phosphorylase a | - |
? | |
| GTP + phosphorylase b | cosubstrate: Mg-UTP complex | Saccharomyces cerevisiae | GDP + phosphorylase a | - |
? | |
| UTP + phosphorylase b | cosubstrate: Mg-UTP complex | Saccharomyces cerevisiae | UDP + phosphorylase a | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 9 | 70% of maximal activity at pH 7.2 and and 9 | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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