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Literature summary for 2.7.11.19 extracted from
- Purification and properties of the cardiac isoenzyme of phosphorylase kinase (1980), J. Biol. Chem., 255, 11794-11801.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| adenosine 3',5'-monophosphate | - |
Bos taurus |  |
| Catalytic subunit of cAMP-dependent protein kinase | activation of nonactivated enzyme | Bos taurus | |
| Catalytic subunit of cAMP-dependent protein kinase | by phosphorylation of alpha' | Bos taurus | |
| Catalytic subunit of cAMP-dependent protein kinase | and beta (not gamma) | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | otherwise activating; total inhibition if ATP concentration exceeds that of divalent cation | Bos taurus | |
| EGTA | Ca2+ restores; strong | Bos taurus | |
| GTP | weak, with ATP as substrate | Bos taurus | |
| ITP | weak, with ATP as substrate | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | 0.22 | ATP | at different phosphorylase b concentrations | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Bos taurus | 5829 | - |
| soluble | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | requirement | Bos taurus | |
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Bos taurus | |
| Mn2+ | requirement | Bos taurus | |
| Mn2+ | can substitute for Mg2+ (less effective) | Bos taurus | |
| phosphate | requirement, phosphate containing enzyme | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
4 * 134000 + 4 * 125000 + 4 * 48000 + 4 * ?, (alphabetagammadelta)4, SDS-PAGE, the forth subunit is comigrating with calmodulin | Bos taurus |
| 125000 | - |
4 * 134000 + 4 * 125000 + 4 * 48000 + 4 * ?, (alphabetagammadelta)4, SDS-PAGE, the forth subunit is comigrating with calmodulin | Bos taurus |
| 134000 | - |
4 * 134000 + 4 * 125000 + 4 * 48000 + 4 * ?, (alphabetagammadelta)4, SDS-PAGE, the forth subunit is comigrating with calmodulin | Bos taurus |
| 1300000 | - |
bovine, sucrose density gradient centrifugation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to near homogeneity | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cardiac muscle | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.68 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, several months | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Bos taurus | ADP + phosphorylase a | - |
? | |
| additional information | ITP, and CTP are no substrates | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexadecamer | 4 * 134000 + 4 * 125000 + 4 * 48000 + 4 * ?, (alphabetagammadelta)4, SDS-PAGE, the forth subunit is comigrating with calmodulin | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 8.8 | progressive increase of activity | Bos taurus |
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