Activating Compound | Comment | Organism | Structure |
---|---|---|---|
adenosine 3',5'-monophosphate | cAMP mediated activation | Rattus norvegicus | |
adenosine 3',5'-monophosphate | cAMP mediated activation | Oryctolagus cuniculus |
General Stability | Organism |
---|---|
unstable in dilute solutions | Rattus norvegicus |
unstable in dilute solutions | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EGTA | - |
Oryctolagus cuniculus | |
EGTA | - |
Rattus norvegicus | |
Mg2+ | in excess of ATP; nonactivated and activated enzyme | Oryctolagus cuniculus | |
Mg2+ | in excess of ATP; nonactivated and activated enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus | |
additional information | - |
additional information | - |
Oryctolagus cuniculus | |
0.07 | - |
MgATP2- | activated rabbit skeletal muscle enzyme, pH 8.2 | Rattus norvegicus | |
0.07 | - |
MgATP2- | activated rabbit skeletal muscle enzyme, pH 8.2 | Oryctolagus cuniculus | |
0.08 | - |
MgATP2- | activated rabbit skeletal muscle enzyme, pH 6 | Rattus norvegicus | |
0.08 | - |
MgATP2- | activated rabbit skeletal muscle enzyme, pH 6 | Oryctolagus cuniculus | |
0.1 | - |
MgATP2- | nonactivated rabbit skeletal muscle enzyme, pH 6 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
soluble | - |
Rattus norvegicus | - |
- |
soluble | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required for efficient substrate binding of active and nonactivated enzyme and for maximal catalysis of active enzyme | Rattus norvegicus | |
Ca2+ | required for efficient substrate binding of active and nonactivated enzyme and for maximal catalysis of active enzyme | Oryctolagus cuniculus | |
Mg2+ | requirement | Rattus norvegicus | |
Mg2+ | requirement | Oryctolagus cuniculus | |
Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Rattus norvegicus | |
Mg2+ | major role of Mg2+: cosubstrate in Mg2+-ATP complex | Oryctolagus cuniculus | |
phosphate | requirement, phosphate containing enzyme | Rattus norvegicus | |
phosphate | requirement, phosphate containing enzyme | Oryctolagus cuniculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
1300000 | - |
rat, gel filtration | Rattus norvegicus |
1300000 | - |
rat, gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
male Wistar | - |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
skeletal muscle | - |
Rattus norvegicus | - |
skeletal muscle | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
-20°C, in 0.25 M sucrose, 0.1 M Tris-HCl, 0.5 mM DTT, pH 7.4, 50% glycerol, several weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycogen synthase | inactivation of skeletal muscle glycogen synthase in the presence or absence of EGTA | Rattus norvegicus | ADP + phosphoglycogen synthase | - |
? | |
ATP + glycogen synthase | inactivation of skeletal muscle glycogen synthase in the presence or absence of EGTA | Oryctolagus cuniculus | ADP + phosphoglycogen synthase | - |
? | |
ATP + glycogen synthase | at high concentration, from rabbit skeletal muscle | Rattus norvegicus | ADP + phosphoglycogen synthase | - |
? | |
ATP + glycogen synthase | at high concentration, from rabbit skeletal muscle | Oryctolagus cuniculus | ADP + phosphoglycogen synthase | - |
? | |
ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Rattus norvegicus | ADP + phosphorylase a | - |
? | |
ATP + phosphorylase b | cosubstrate: Mg-ATP complex | Oryctolagus cuniculus | ADP + phosphorylase a | - |
? | |
ATP + phosphorylase b | rabbit skeletal muscle | Rattus norvegicus | ADP + phosphorylase a | - |
? | |
ATP + phosphorylase b | rabbit skeletal muscle | Oryctolagus cuniculus | ADP + phosphorylase a | - |
? | |
additional information | histone H2B is no substrate | Rattus norvegicus | ? | - |
? | |
additional information | histone H2B is no substrate | Oryctolagus cuniculus | ? | - |
? | |
additional information | no spontaneous or MnSO4-induced dephosphorylation of activated enzyme | Rattus norvegicus | ? | - |
? | |
additional information | no spontaneous or MnSO4-induced dephosphorylation of activated enzyme | Oryctolagus cuniculus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
nonactivated enzyme | Rattus norvegicus |
7 | - |
- |
Oryctolagus cuniculus |
7 | - |
above, activated enzyme | Rattus norvegicus |