Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | required | Mus musculus |
Cloned (Comment) | Organism |
---|---|
gene Mylk, the MLCK locus encodes three transcripts from alternative promoters and produces two MLCK isoforms (short MLCK and long MLCK) and a C-terminal Ig module (telokin). Long MLCK (L-MLCK) is identical to short MLCK (S-MLCK) apart from a unique N-terminal extension containing two extra DFRXXL motifs and six Ig modules | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of enzyme MLCK deletion in intestinal smooth muscle cells, phenotype, overview | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Mus musculus | |
Mg2+ | required | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | Mus musculus | non-muscle myosin II and preferred substrate smooth muscle myosin II | ADP + [myosin light chain] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q6PDN3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Mus musculus | - |
smooth muscle cell | primary cultured cells | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | non-muscle myosin II and preferred substrate smooth muscle myosin II | Mus musculus | ADP + [myosin light chain] phosphate | - |
? | |
ATP + [myosin light chain] | phosphorylation of Thr-18/Ser-19 on the regulatory light chain of myosin II | Mus musculus | ADP + [myosin light chain] phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the MLCK locus encodes three transcripts from alternative promoters and produces two MLCK isoforms (short MLCK and long MLCK) and a C-terminal Ig module (telokin). Long MLCK (L-MLCK) is identical to short MLCK (S-MLCK) apart from a unique N-terminal extension containing two extra DFRXXL motifs and six Ig modules | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
MLCK | - |
Mus musculus |
myosin light chain kinase | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | MLCK activates myosin Mg-ATPase activity by phosphorylating Thr-18/Ser-19 on the RLC of myosin II, thereby initiating cross-bridge movements of both smooth muscle and non-muscle. MLCK deletion causes enhanced cell protrusion along with a reduction of membrane tension and is rescued by kinase-dead MLCK or five-DFRXXL motif. The deletion of MLCK results in fast cell migration, enhanced protrusion formation, but no alteration of myosin light chain phosphorylation. The mutant cells show reduced membrane tether force and fewer membrane F-actin filaments, as well as a higher average migrating velocity and protrusion compared to wild-type | Mus musculus |
physiological function | MLCK regulates cell migration not by myosin regulatory light chain phosphorylation but possibly through a membrane tension-based mechanism, which is a distinct regulatory mechanism of protrusion during cell migration. MLCK is not required for myosin phosphorylation in a migrating cell, but negatively controls migration by maintaining membrane tension. A critical role of MLCK in cell migration involves regulating the cell membrane tension and protrusion necessary for migration, thereby stabilizing the membrane skeleton through F-actin-binding activity. MLCK regulates the membrane F-actin skeleton. MLCK regulates cell spreading through 5DFRXXL in a kinase-independent manner | Mus musculus |