Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on, antagonizes the binding to actin | Gallus gallus | |
Calmodulin | dependent on, antagonizes the binding to actin, residues 26-41 of MLCK act as a CaM-binding region that regulates the binding of MLCK to actin, Ca/CaM is also binds at the Ala796-Ser815 sequence to regulate the kinase activity MLCK | Bos taurus | |
additional information | oxytoxin and phorbol ester do not affect the enzyme activity | Gallus gallus |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Gallus gallus |
expression of wild-type full-length MLCK and of several enzyme fragments in Escherichia coli with the use of a cold-shock promoter | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | downregulation of MLCK in cultured smooth muscle cells reveals that MLC20 phosphorylation is not obligatory for the smooth muscle to contract, overview | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | non-kinase, inhibitory activity of N-terminal, actin-binding domain of MLCK | Bos taurus | |
additional information | oxytoxin and phorbol ester do not affect the enzyme activity. Non-kinase, inhibitory activity of N-terminal, actin-binding domain of MLCK | Gallus gallus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, antagonizes the binding to actin | Gallus gallus | |
Ca2+ | dependent on, antagonizes the binding to actin, Ca/CaM binds at the Ala796-Ser815 sequence to regulate the kinase activity MLCK | Bos taurus | |
Mg2+ | - |
Gallus gallus | |
Mg2+ | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
- |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin light chain | Gallus gallus | - |
ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | Bos taurus | - |
ADP + myosin light chain phosphate | - |
? | |
additional information | Bos taurus | actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview | ? | - |
? | |
additional information | Gallus gallus | actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview. MLCK phosphorylated at Ser828 stimulates the ATPase activity of unphosphorylated myosin more effectively than the control MLCK | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Gallus gallus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | protein kinase A phosphorylates MLCK in its regulatory domain at Ser1021 and Ser1034. The kinase activity of MLCK phosphorylated at Ser1021 is reduced, whereas that of MLCK phosphorylated at Ser1034 is not reduced | Bos taurus |
phosphoprotein | protein kinase A phosphorylates MLCK in its regulatory domain at Ser815 and Ser828. The kinase activity of MLCK phosphorylated at Ser815 is reduced, whereas that of MLCK phosphorylated at Ser828 is not reduced | Gallus gallus |
Purification (Comment) | Organism |
---|---|
native enzyme from gizzard muscle | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cardiac muscle | - |
Gallus gallus | - |
cardiac muscle | - |
Bos taurus | - |
skeletal muscle | - |
Gallus gallus | - |
skeletal muscle | - |
Bos taurus | - |
smooth muscle | from gizzard | Gallus gallus | - |
smooth muscle | from stomach | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin light chain | - |
Gallus gallus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | - |
Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | the enzyme has effects on the actin-myosin interaction in vitro, binding of full-length and fragmented enzyme, overview. The Asp-Phe-Arg-X-X-Leu motif plays a major role in the binding of MLCK to myofibrils. Regulation of actinmyosin interaction by MLCK through its non-kinase activity, overview | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | the enzyme has effects on the actin-myosin interaction in vitro, regulation of actin-myosin interaction by MLCK through its non-kinase activity, overview | Gallus gallus | ADP + myosin light chain phosphate | - |
? | |
additional information | actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview | Bos taurus | ? | - |
? | |
additional information | actin-myosin interaction of smooth muscle could be activated by the non-kinase activity of MLCK, a mechanism that is quite independent of MLC20 phosphorylation, role of non-kinase activity of myosin light-chain kinase in regulating smooth muscle contraction, overview. MLCK phosphorylated at Ser828 stimulates the ATPase activity of unphosphorylated myosin more effectively than the control MLCK | Gallus gallus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | MLCK domain structure, a fusion protein consisting of the N-terminal actin-binding domain, the central catalytic domain, and the C-terminal myosin-binding domain | Bos taurus |
More | MLCK domain structure, a fusion protein consisting of the Nterminal actin-binding domain, the central catalytic domain, and the C-terminal myosin-binding domain | Gallus gallus |
Synonyms | Comment | Organism |
---|---|---|
MLCK | - |
Gallus gallus |
MLCK | - |
Bos taurus |
myosin light chain kinase | - |
Gallus gallus |
myosin light chain kinase | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Gallus gallus | |
ATP | ATP-binding motif X-Gly-X-Gly-X-XGly-X | Bos taurus |