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Literature summary for 2.7.11.18 extracted from

  • Wadgaonkar, R.; Dudek, S.M.; Zaiman, A.L.; Linz-McGillem, L.; Verin, A.D.; Nurmukhambetova, S.; Romer, L.H.; Garcia, J.G.
    Intracellular interaction of myosin light chain kinase with macrophage migration inhibition factor (MIF) in endothelium (2005), J. Cell. Biochem., 95, 849-858.
    View publication on PubMed
Search for more literature for 2.7.11.18

Activating Compound

Activating Compound Comment Organism Structure
additional information the endothelial isozyme EC MLCK contains a unique N-terminal sequence which is a target for upregulation by signaling cascade components such as p60src Homo sapiens
thrombin stimulates endothelial isozyme activity in endothelium, and increases EC MLCK interaction with macrophage migration inhibition factor MIF Homo sapiens
TNFalpha stimulates endothelial isozyme activity in endothelium, and increases EC MLCK interaction with macrophage migration inhibition factor MIF Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of epitope-tagged endothelial isozyme in endothelial cells, expression of GST-fusion isozyme EC MLCK in bacteria, expression of endothelial iszyme EC MLCK and macrophage migration inhibition factor MIF in the yeast two-hybrid system, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [myosin light chain] Homo sapiens the endothelial isozyme is a mutifunctional contractile effector involved in vascular barrier regulation, leukocyte diapedesis, apoptosis, and angiogenesis ADP + [myosin light chain] phosphate
-
 ?
additional information Homo sapiens the enzyme interacts with macrophage migration inhibition factor MIF in endothelium which may be important for the regulation of both non-muscle cytoskeletal dynamics as well as pathobiological vascular events involving the enzyme ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 endothelial isozyme EC MLCK exists in different splice variants
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion isozyme EC MLCK by affinity chromatgraphy Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
endothelium endothelial isozyme EC MLCK Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [myosin light chain] the endothelial isozyme is a mutifunctional contractile effector involved in vascular barrier regulation, leukocyte diapedesis, apoptosis, and angiogenesis Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
ATP + [myosin light chain] activated phosphorylated enzyme Homo sapiens ADP + [myosin light chain] phosphate
-
 ?
additional information the enzyme interacts with macrophage migration inhibition factor MIF in endothelium which may be important for the regulation of both non-muscle cytoskeletal dynamics as well as pathobiological vascular events involving the enzyme Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More the endothelial isozyme EC MLCK contains a unique N-terminal sequence which is a target for upregulation by signaling cascade components such as p60src, EC MLCK splice variants doamin structure Homo sapiens

Synonyms

Synonyms Comment Organism
EC MLCK
-
 Homo sapiens
endothelial cell myosin light chain kinase
-
 Homo sapiens
myosin light chain kinase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens
Calmodulin
-
 Homo sapiens