Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | 140fold activation through phosphorylation at Thr166 | Dictyostelium discoideum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | residues 283-288 function as an autoinhibitory domain, autoinhibition is fully relieved by Thr166 phosphorylation | Dictyostelium discoideum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Dictyostelium discoideum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dictyostelium discoideum | P25323 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme is 140fold activated through phosphorylation at Thr166 by a recombinant constitutively active Ca2+/calmodulin-dependent protein kinase kinase, the enzyme performs autophosphorylation at Thr289, subsequent to Thr166 phosphorylation by the protein kinase kinase, and at several serine residues, leading to release of the autoinhibitory domain from the catalytic core, identification of phosphorylation sites | Dictyostelium discoideum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + kemptamide | i.e. peptide KKRPQRATSNVFS-NH2, activated enzyme | Dictyostelium discoideum | ADP + kemptamide phosphate | - |
? | |
ATP + myosin II | from Dictyostelium discoideum, activated enzyme | Dictyostelium discoideum | ADP + myosin II phosphate | - |
? | |
ATP + [myosin II light chain] | from Dictyostelium discoideum, activated enzyme | Dictyostelium discoideum | ADP + [myosin II lihght chain] phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MLCK-A | - |
Dictyostelium discoideum |
myosin light chain kinase A | - |
Dictyostelium discoideum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Dictyostelium discoideum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Dictyostelium discoideum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Dictyostelium discoideum |