Cloned (Comment) | Organism |
---|---|
expression of a C-terminal enzyme fragment comprising residues 860-1176 as GST-fusion protein in Escherichia coli strain Bl21(DE3), the GST-tag slightly reduces the activity of the recombinant enzyme fragment compared to the untagged fragment | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | complex formation with calmodulin | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | Bos taurus | the multifunctional regulatory enzyme stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain, kinetics, overview | ADP + [myosin light chain] phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged C-terminal enzyme fragment by glutathione affinity chromatography | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
smooth muscle | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [myosin light chain] | - |
Bos taurus | ADP + [myosin light chain] phosphate | - |
? | |
ATP + [myosin light chain] | the multifunctional regulatory enzyme stimulates smooth muscle myosin ATPase activity by binding to the myosin heads without phosphorylating the myosin light chain, kinetics, overview | Bos taurus | ADP + [myosin light chain] phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MLCK | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus | |
Calmodulin | binding site I at residue 1002-1022, binding site II at residues 26-42, complex formation with Ca2+ | Bos taurus |