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Literature summary for 2.7.11.18 extracted from

  • Sellers, J.R.; Harvey, E.V.
    Purification of myosin light chain kinase from Limulus muscle (1984), Biochemistry, 23, 5821-5826.
    View publication on PubMed
Search for more literature for 2.7.11.18

Activating Compound

Activating Compound Comment Organism Structure
autophosphorylation no activation Limulus sp.
phosphorylation no activation Limulus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0156
-
 Limulus myosin light chain 25°C, pH 7.5 Limulus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Limulus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 PAGE, 2 forms of myosin light chain kinase Limulus sp.
39000
-
 PAGE, 2 forms of myosin light chain kinase Limulus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + myosin light chain Limulus sp.
-
 ADP + myosin light chain phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Limulus sp.
-
 horseshoe crab
-

Purification (Commentary)

Purification (Comment) Organism
-
 Limulus sp.

Source Tissue

Source Tissue Comment Organism Textmining
telson muscle Limulus sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + myosin light chain
-
 Limulus sp. ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain no substrates are actin and tropomyosin Limulus sp. ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain no substrate is histone 2-A Limulus sp. ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain no substrate is casein Limulus sp. ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain no substrate is histone 2 b Limulus sp. ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain no substrate is histone H1 Limulus sp. ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain 1 mol phosphate per mol light chain in skeletal muscle Limulus sp. ADP + myosin light chain phosphate
-
 ?
ATP + myosin light chain specific for 20 kDa light chain Limulus sp. ADP + myosin light chain phosphate
-
 ?
additional information no intramolecular autophosphorylation in the absence of acceptor substrate Limulus sp. ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, SDS-PAGE 2 forms of myosin light chain kinase Limulus sp.
monomer 1 * 39000, SDS-PAGE, 2 forms of myosin light chain kinase Limulus sp.

Cofactor

Cofactor Comment Organism Structure
Calmodulin requirement Limulus sp.