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Literature summary for 2.7.11.18 extracted from
- Purification of smooth muscle myosin light-chain kinase (1982), Methods Enzymol., 85, 298-308.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| protease inhibitors stabilize during purification | Meleagris gallopavo |
| protease inhibitors stabilize during purification | Homo sapiens |
| repeated freeze-thawing decreases activity | Meleagris gallopavo |
| repeated freeze-thawing decreases activity | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
myosin light chain | - |
Meleagris gallopavo |  |
| 0.05 | - |
ATP | - |
Meleagris gallopavo | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| myofibril | associated | Meleagris gallopavo | 30016 | - |
| myofibril | associated | Homo sapiens | 30016 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Meleagris gallopavo | |
| Ca2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 124000 | - |
sedimentation equilibrium centrifugation | Meleagris gallopavo |
| 130000 | - |
1 * 130000, SDS-PAGE | Meleagris gallopavo |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Meleagris gallopavo | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Homo sapiens | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Meleagris gallopavo | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Meleagris gallopavo |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| platelet | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Meleagris gallopavo |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | - |
Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle myosin | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle myosin | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | transfers the gamma-phosphate of ATP to a Ser-residue of myosin light chain | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | not 16 kDa light chain | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Homo sapiens |
| monomer | 1 * 130000, SDS-PAGE | Meleagris gallopavo |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Meleagris gallopavo | |
| Calmodulin | requirement | Homo sapiens | |
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Release 2023.1 (January 2023)
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