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Literature summary for 2.7.11.18 extracted from
- Purification and characterization of smooth muscle myosin light chain kinase (1981), J. Biol. Chem., 256, 7501-7509.
General Stability
| General Stability | Organism |
|---|---|
| EGTA prevents Ca2+-dependent proteolysis during initial purification | Meleagris gallopavo |
| MgCl2 is critical for kinase extraction from myofibrils | Meleagris gallopavo |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
myosin light chain | 24°C, pH 7.3 | Meleagris gallopavo |  |
| 0.05 | - |
ATP | 24°C, pH 7.3 | Meleagris gallopavo | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| myofibril | associated | Meleagris gallopavo | 30016 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Meleagris gallopavo | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Meleagris gallopavo |
| 124000 | - |
sedimentation equilibrium centrifugation | Meleagris gallopavo |
| 130000 | - |
gel filtration | Meleagris gallopavo |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Meleagris gallopavo | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Meleagris gallopavo | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| affinity chromatography on calmodulin-Sepharose | Meleagris gallopavo |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gizzard | - |
Meleagris gallopavo | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Meleagris gallopavo |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, 30% loss of activity within 3 weeks | Meleagris gallopavo |
| -70°C, at least 6 months | Meleagris gallopavo |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | - |
Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle myosin | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2-A | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase kinase | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone V-S | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 130000, SDS-PAGE | Meleagris gallopavo |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 24 | - |
assay at | Meleagris gallopavo |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Meleagris gallopavo | |
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