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Literature summary for 2.7.11.18 extracted from
- Purification and characterization of myosin light-chain kinase from the rat pancreas (1989), Biochem. J., 258, 739-747.
General Stability
| General Stability | Organism |
|---|---|
| protease inhibitors stabilize during purification | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000002 | - |
Calmodulin | 30°C, pH 7.5 | Rattus norvegicus |  |
| 0.018 | - |
myosin light chain | 30°C, pH 7.5 | Rattus norvegicus | |
| 0.073 | - |
ATP | 30°C, pH 7.5 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | KM: 0.0025 mM | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 138000 | - |
x * 138000, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Rattus norvegicus | involved in myosin phosphorylation and enzyme secretion | ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | rat pancreatic and turkey gizzard enzyme are immunologically related | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.47 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 1% bovine serum albumin, more than 2 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | no substrate is histone 2-A | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for 18 kDa light chain | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is synapsin | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | involved in myosin phosphorylation and enzyme secretion | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 138000, SDS-PAGE | Rattus norvegicus |
| monomer | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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