Activating Compound | Comment | Organism | Structure |
---|---|---|---|
autophosphorylation | no activation | Sus scrofa | |
additional information | no activation by phosphatidylserine | Sus scrofa | |
additional information | no activation by cAMP | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cAMP-dependent protein kinase | phosphorylates light chain myosin kinase leading to decreased affinity from calmodulin | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | 0.027 | turkey gizzard myosin light chain | 30°C, pH 7 | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Sus scrofa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Km-value: 0.0003 | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
130000 | - |
x * 130000, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin light chain | Sus scrofa | - |
ADP + myosin light chain phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
myometrium | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.9 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, quite unstable on storage | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin light chain | - |
Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrates are myosin heavy chain and phosvitin | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is protamine | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is casein | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | highly specific for regulatory or P-light-chain | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | highly specific for 18 kDa light chain | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is phosphorylase kinase | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | specific for 20 kDa light chain | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
ATP + myosin light chain | no substrate is phosphorylase b | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
additional information | no intramolecular autophosphorylation in the absence of acceptor substrate | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 130000, SDS-PAGE | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | requirement | Sus scrofa | |
Calmodulin | lower affinity for Ca2+/caldesmon after phosphorylation by cAMP-dependent protein kinase, Ka: 0.0000006 mM | Sus scrofa |