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Literature summary for 2.7.11.18 extracted from
- Purification and characterization of pregnant sheep myometrium myosin light chain kinase (1991), Arch. Biochem. Biophys., 287, 24-32.
General Stability
| General Stability | Organism |
|---|---|
| protease inhibitors stabilize during purification | Meleagris gallopavo |
| protease inhibitors stabilize during purification | Homo sapiens |
| protease inhibitors stabilize during purification | Ovis aries |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
turkey gizzard myosin light chain | pH 7.5, myometrium enzyme | Ovis aries |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Meleagris gallopavo | |
| Ca2+ | - |
Homo sapiens | |
| Ca2+ | - |
Ovis aries | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Meleagris gallopavo | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Homo sapiens | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Ovis aries | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Meleagris gallopavo | - |
- |
- |
| Ovis aries | - |
pregnant and non-pregnant | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ovis aries |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bladder | - |
Ovis aries | - |
| gizzard | - |
Meleagris gallopavo | - |
| gizzard | pregnant sheep myometrium, turkey and chicken gizzard enzyme are immunologically related | Ovis aries | - |
| myometrium | pregnant sheep myometrium, turkey and chicken gizzard enzyme are immunologically related | Ovis aries | - |
| stomach | - |
Meleagris gallopavo | - |
| stomach | - |
Ovis aries | - |
| trachea | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
rabbit uterine myosine | Ovis aries |
| 7.7 | - |
turkey gizzard myosine | Ovis aries |
Storage Stability
| Storage Stability | Organism |
|---|---|
| proteolysis occurs even on storage at -80°C, myometrium enzyme | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | - |
Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Ovis aries | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2-A | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2-A | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2-A | Ovis aries | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Ovis aries | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Ovis aries | ADP + myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | SDS-PAGE | Ovis aries |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Meleagris gallopavo |
| 22 | - |
assay at | Homo sapiens |
| 22 | - |
assay at | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Meleagris gallopavo | |
| Calmodulin | requirement | Homo sapiens | |
| Calmodulin | requirement | Ovis aries | |
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