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Literature summary for 2.7.11.18 extracted from
- Purification of caldesmon and myosin light chain (MLC) kinase from arterial smooth muscle: comparisons with gizzard caldesmon and MLC kinase (1987), J. Biochem., 101, 1-9.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | 0.0095 | myosin light chain | - |
Gallus gallus |  |
| 0.005 | 0.0095 | myosin light chain | 30°C, pH 7.2 | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| actin filament | F-actin-associated along cellular stress fibres | Bos taurus | 5884 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Gallus gallus | |
| Ca2+ | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 135000 | - |
x * 135000, SDS-PAGE | Gallus gallus |
| 160000 | - |
x * 160000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Gallus gallus | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Bos taurus | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Gallus gallus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| gizzard | - |
Gallus gallus | - |
| smooth muscle | - |
Gallus gallus | - |
| smooth muscle | arterial | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | - |
Gallus gallus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2-A | Gallus gallus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2-A | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Gallus gallus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle | Gallus gallus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone V-S | Gallus gallus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone V-S | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Gallus gallus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 160000, SDS-PAGE | Bos taurus |
| ? | x * 135000, SDS-PAGE | Gallus gallus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Gallus gallus | |
| Calmodulin | requirement | Bos taurus | |
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Release 2023.1 (January 2023)
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