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Literature summary for 2.7.11.18 extracted from
- Purification, characterization and substrate specificity of calmodulin-dependent myosin light-chain kinase from bovine brain (1987), Biochem. J., 247, 747-756.
General Stability
| General Stability | Organism |
|---|---|
| glycerol and Tween 40 stabilize | Bos taurus |
| unstable upon lyophilization | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cAMP-dependent protein kinase | phosphorylates light chain myosin kinase leading to decreased affinity from calmodulin | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000002 | - |
Calmodulin | 30°C, pH 6.8 | Bos taurus |  |
| 0.02 | 0.027 | turkey gizzard myosin light chain | 30°C, pH 6.8 | Bos taurus | |
| 0.11 | - |
kemptamide | 30°C, pH 6.8 | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Bos taurus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bos taurus | |
| Mg2+ | Km-value: 2 mM | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 127000 | - |
sucrose density gradient centrifugation | Bos taurus |
| 152000 | - |
x * 152000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Bos taurus | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
myosin light chain, isolated | Bos taurus |
| 2.51 | - |
myosin light chain, in native myosin | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + kemptamide | - |
Bos taurus | ADP + ? | - |
? | |
| ATP + myosin light chain | - |
Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2 b | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for regulatory or P-light-chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for 18 kDa light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is synapsin | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrates are myelin basic protein, glycogen synthase, tubulin, microtubule-associated protein 2, kemptide and peptide pp60src | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 152000, SDS-PAGE | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | - |
- |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Bos taurus | |
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Release 2023.1 (January 2023)
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