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Literature summary for 2.7.11.18 extracted from
- Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity (1979), Proc. Natl. Acad. Sci. USA, 76, 1653-1657.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.018 | - |
myosin light chain | 23-25°C, pH 7.5 | Homo sapiens | |
| 0.121 | - |
ATP | 23-25°C, pH 7.5 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| actomyosin | associated | Homo sapiens | 42641 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
x * 105000, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Homo sapiens | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, affinity chromatography on calmodulin-Sepharose | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| platelet | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.1 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | - |
Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrates are myosin heavy chain and phosvitin | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is protamine | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2-A | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of smooth muscle | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone V-S | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 105000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 25 | assay at | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.17 | - |
myosin light chain | 23-25°C, pH 7.5 | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Homo sapiens | |
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Release 2023.1 (January 2023)
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