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Literature summary for 2.7.11.17 extracted from

  • Gentzel, M.; Schille, C.; Rauschenberger, V.; Schambony, A.
    Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation (2015), Mol. Biol. Cell, 26, 966-977.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of CAMKIIalpha Xenopus laevis
recombinant expression of CAMKIIbeta Xenopus laevis
transient expression of CAMKIIdelta in HEK-293T cells, pull-down assay by FLAG-tagged proteins Arrb2 and Dvl2 Xenopus laevis
transient expression of CAMKIIgamma in HEK-293T cells, pull-down assay by FLAG-tagged proteins Arrb2 and Dvl2 Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis Q6AX64
-
-
Xenopus laevis Q91549
-
-
Xenopus laevis Q9DG00
-
-
Xenopus laevis Q9DG02
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Xenopus laevis
-

Synonyms

Synonyms Comment Organism
Ca2+/calmodulin-dependent protein kinase 2
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 Xenopus laevis
CaMKII
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 Xenopus laevis
CaMKIIalpha
-
 Xenopus laevis
CaMKIIbeta
-
 Xenopus laevis
CaMKIIdelta
-
 Xenopus laevis
CaMKIIgamma
-
 Xenopus laevis

General Information

General Information Comment Organism
physiological function in early Xenopus embryos, CamKII contributes to the establishment of the dorsoventral body axis. During gastrula­tion, CamKII regulates the separation of mesoderm and ectoderm and is required for convergent extension movements of the dorsal mesoderm. CamKII isoforms interact differentially with the three vertebrate dishevelled proteins, detailed overview. CamKII interacts with dishevelled Dvl2 but not with Dvl1, and Dvl2 is necessary to mediate CamKII function downstream of Dvl1 in convergent extension movements in Xenopus gastru­lation. Dvl1 is required for the activation of CamKII and PKC in the wingless/integrated-1 protein (Wnt)/Ca2+ pathway. CamKII preferentially interacts with Dvl2, and Dvl2 strongly interacts with CamKIIdelta and additionally with CamKIIbeta and CamKIIalpha. CamKIIgamma does not interact with any Dvl isoform and Dvl1 does not interact with any of the Cam­KII isoforms. Interaction between endogenous CamKII and Dvl3 occurs only after Wnt-5a stimula­tion and at a much lower level than in Dvl2 immunoprecipitates Xenopus laevis
physiological function in early Xenopus embryos, CamKII contributes to the establishment of the dorsoventral body axis. During gastrula­tion, CamKII regulates the separation of mesoderm and ectoderm and is required for convergent extension movements of the dorsal mesoderm. CamKII isoforms interact differentially with the three vertebrate dishevelled proteins, detailed overview. CamKII interacts with dishevelled Dvl2 but not with Dvl1, and Dvl2 is necessary to mediate CamKII function downstream of Dvl1 in convergent extension movements in Xenopus gastru­lation. Dvl1 is required for the activation of CamKII and PKC in the wingless/integrated-1 protein (Wnt)/Ca2+ pathway. CamKII preferentially interacts with Dvl2, and Dvl2 strongly interacts with CamKIIdelta and additionally with CamKIIbeta and CamKIIalpha. CamKIIgamma does not interact with any Dvl isoform. and Dvl1 does not interact with any of the Cam­KII isoforms. Interaction between endogenous CamKII and Dvl3 occurs only after Wnt-5a stimula­tion and at a much lower level than in Dvl2 immunoprecipitates. The protein complex of Arrb2-Dvl2 and CamKIIdelta regulates convergent extension movements. Enhanced CamKII autophos­phorylation in Dvl3-morphant embryos, the convergent extension phenotype in Dvl3-depleted explants is rescued by overexpression of dnCamKIIdelta. In Dvl1/Dvl2 double-morphant embryos, coexpression of CamKIIdelta restores elongation but not constriction of the explants, a pheno­type highly reminiscent of the single Dvl2-knockdown phenotype Xenopus laevis