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Literature summary for 2.7.11.17 extracted from

  • Medvedev, S.; Stein, P.; Schultz, R.M.
    Specificity of calcium/calmodulin-dependent protein kinases in mouse egg activation (2014), Cell Cycle, 13, 1482-1488.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.17

Cloned(Commentary)

Cloned (Comment) Organism
gene Camk2g, recombinant expression in isozyme CaMKIIgamma-deficient mouse eggs and complementation by 80%, quantitative real-time RT-PCR expression analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
K43M site-directed mutagenesis, catalytically inactive form of CaMKIIgamma Mus musculus
additional information generation of isozyme-deficient Camk2gamma-/- eggs, exogenous expression of different cRNAs in the mutant eggs shows that the other multifunctional CaM kinases, CaMKI, and CaMKIV, are not capable of substituting CaMKIIgamma to initiate cell cycle resumption in response to a rise in intracellular Ca2+. Exogenous expression of Camk2g or Camk2d results in activation of nearly 80% of Camk2g-/- MII eggs after stimulation with SrCl2. None of the Camk2g-/- MII eggs expressing Camk1 or Camk4 activate in response to SrCl2 treatment. Expression of a constitutively active form of Camk4 (ca-Camk4), but not Camk1, triggers egg activation Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Emi2 Mus musculus an APC/C repressor, inactivation ADP + phosphorylated Emi2
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 ?
additional information Mus musculus in terms of substrate specificity, the consensus sequences for phosphorylation by CaMKI, CaMKII, and CaMKIV are quite similar, and hence these kinases sometimes phosphorylate the same substrates, for example cAMP-response element-binding protein. Expression of Camk2g and Camk2d, but not Camk1 or Camk4, in Camk2g-/- eggs leads to degradation of exogenously expressed EMI2 ?
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 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q923T9
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Source Tissue

Source Tissue Comment Organism Textmining
additional information Camk2g is expressed in many tissues Mus musculus
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oocyte CaMKIIgamma is the predominant CaMKII isoform in mouse eggs Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Emi2 an APC/C repressor, inactivation Mus musculus ADP + phosphorylated Emi2
-
 ?
additional information in terms of substrate specificity, the consensus sequences for phosphorylation by CaMKI, CaMKII, and CaMKIV are quite similar, and hence these kinases sometimes phosphorylate the same substrates, for example cAMP-response element-binding protein. Expression of Camk2g and Camk2d, but not Camk1 or Camk4, in Camk2g-/- eggs leads to degradation of exogenously expressed EMI2 Mus musculus ?
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 ?
additional information CaMKIIgamma possesses an intrinsic autophosphorylating activity, which renders the enzyme insensitive to changes in intracellular Ca2+ level Mus musculus ?
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 ?

Subunits

Subunits Comment Organism
More CaMKII is a 12-subunit holoenzyme comprised of homo- or heteromers of alpha, beta, gamma, and delta subunits encoded by 4 different genes. The CaMK family shares considerable sequence homology and domain structure. They all have an N-terminal catalytic domain and a central auto-inhibitory and Ca2+/CaM-binding domain. CaMKII also has a unique C-terminal association domain involved in multimerization of the holoenzyme Mus musculus

Synonyms

Synonyms Comment Organism
calcium/calmodulin-dependent protein kinase
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 Mus musculus
Camk2g
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 Mus musculus
CaMKIIgamma
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 Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus

General Information

General Information Comment Organism
malfunction exogenous expression of different cRNAs in isozyme-deficient Camk2gamma-/- eggs shows that the other multifunctional CaM kinases, CaMKI, and CaMKIV, are not capable of substituting CaMKIIgamma to initiate cell cycle resumption in response to a rise in intracellular Ca2+. Exogenous expression of Camk2g or Camk2d results in activation of nearly 80% of Camk2g-/- MII eggs after stimulation with SrCl2. None of the Camk2g-/- MII eggs expressing Camk1 or Camk4 activate in response to SrCl2 treatment. Expression of catalytically inactive CaMKIIgamma mutant K43M in Camk2g-/- eggs to the same level as wild-type Camk2g does not result in EMI2 degradation. Expression of a constitutively active form of Camk4 (ca-Camk4), but not Camk1, triggers egg activation. Female mice lacking CaMKIIgamma are infertile, due to their inability to exit the metaphase II arrest, but they appear morphologically normal Mus musculus
physiological function CaMKIIgamma, the predominant CaMKII isoform in mouse eggs, controls egg activation by regulating cell cycle resumption. Degradation of EMI2 following its phosphorylation specifically by CaMKII is mechanistically linked to and promotes cell cycle resumption in MII eggs Mus musculus