Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIalpha; product inhibition is best fit with an ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIbeta; product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIdelta; product inhibition is best fit with an ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus ATP, uncompetitive inhibition versus phospholamban, isoenzyme CaMK IIgamma | Homo sapiens | |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIalpha; product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIbeta; product inhibition is best fit with an Ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIdelta; product inhibition is best fit with an ordered Bi Bi mechanism in which phospholamban is the first substrate to bind and ADP is the last product to be released. Competitive inhibition versus phospholamban and ATP, isoenzyme CaMK IIgamma | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0064 | - |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | pH 7.6, 25°C, isoenzyme CaMK IIdelta | Homo sapiens | |
0.0071 | - |
ATP | pH 7.6, 25°C, isoenzyme CaMK IIalpha | Homo sapiens | |
0.0072 | - |
ATP | pH 7.6, 25°C, isoenzyme CaMK IIdelta | Homo sapiens | |
0.0084 | - |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | pH 7.6, 25°C, isoenzyme CaMK IIgamma | Homo sapiens | |
0.0097 | - |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | pH 7.6, 25°C, isoenzyme CaMK IIalpha | Homo sapiens | |
0.0128 | - |
ATP | pH 7.6, 25°C, isoenzyme CaMK IIgamma | Homo sapiens | |
0.0158 | - |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | pH 7.6, 25°C, isoenzyme CaMK IIbeta | Homo sapiens | |
0.0259 | - |
ATP | pH 7.6, 25°C, isoenzyme CaMK IIbeta | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + phospholamban | Homo sapiens | - |
ADP + phosphorylated phospholamban | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13554 | - |
- |
Homo sapiens | Q13555 | - |
- |
Homo sapiens | Q13557 | - |
- |
Homo sapiens | Q9UQM7 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | sequential reaction mechanism in which all substrates bind to enzyme before any product is released. Binding of phospholamban to the enzyme decreases the affinity of the enzyme toward ATP | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | biotinylated phospholamban Thr17 peptide substrate | Homo sapiens | ADP + biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-phospho-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | - |
? | |
ATP + phospholamban | - |
Homo sapiens | ADP + phosphorylated phospholamban | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CaMK IIalpha | - |
Homo sapiens |
CaMK IIbeta | - |
Homo sapiens |
CaMK IIdelta | - |
Homo sapiens |
CaMK IIgamma | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
ATP | pH 7.6, 25°C, isoenzyme CaMK IIalpha | Homo sapiens | |
1.2 | - |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | pH 7.6, 25°C, isoenzyme CaMK IIalpha | Homo sapiens | |
8 | - |
ATP | pH 7.6, 25°C, isoenzyme CaMK IIbeta | Homo sapiens | |
8 | - |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | pH 7.6, 25°C, isoenzyme CaMK IIbeta | Homo sapiens | |
9.8 | - |
ATP | pH 7.6, 25°C, isoenzyme CaMK IIgamma | Homo sapiens | |
9.8 | - |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | pH 7.6, 25°C, isoenzyme CaMK IIgamma | Homo sapiens | |
9.9 | - |
ATP | pH 7.6, 25°C, isoenzyme CaMK IIdelta | Homo sapiens | |
9.9 | - |
biotinylated Thr-Arg-Ser-Ala-Ile-Arg-Arg-Ala-Ser-Thr-Ile-Glu-Met-Pro-Gln-Gln-Ala-Arg-Gln | pH 7.6, 25°C, isoenzyme CaMK IIdelta | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Homo sapiens |