Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on | Rattus norvegicus | |
additional information | CaM-K Ialpha is activated in vitro by phosphorylation through kinase CaM-KK at Thr177 independently of Ca2+ and calmodulin | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of HA-tagged wild-type CaM-KIalpha, CaM-K IV, and CaM-K IIalpha, and CaM-K Ialpha mutant K49A, and constitutively active CaM-K Ialpha truncation mutant residues 1-293 in HEK293 cells, Escherichia coli, and Sf9 insect cells | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Rattus norvegicus | |
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + neuronal nitric-oxide synthase | Rattus norvegicus | phosphorylation at Ser741 by CaM-K Ialpha, wild-type and truncation mutant 1-293 inhibits neuronal nitric-oxide synthase, nNOS, no substrate of CaM-K IIalpha and CaM-K IV | ADP + phosphorylated neuronal nitric-oxide synthase | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | CaM-K performs autophosphorylation, CaM-K Ialpha is activated in vitro by phosphorylation through kinase CaM-KK at Thr177 independently of Ca2+ and calmodulin | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + neuronal nitric-oxide synthase | phosphorylation at Ser741 by CaM-K Ialpha, wild-type and truncation mutant 1-293 inhibits neuronal nitric-oxide synthase, nNOS, no substrate of CaM-K IIalpha and CaM-K IV | Rattus norvegicus | ADP + phosphorylated neuronal nitric-oxide synthase | - |
? | |
ATP + neuronal nitric-oxide synthase | phosphorylation at Ser741 by CaM-K Ialpha, wild-type and truncation mutant 1-293 inhibits neuronal nitric-oxide synthase, nNOS, no substrate of CaM-K IIalpha and CaM-K IV, determination of phosphorylation sites by analytical SDS-PAGE | Rattus norvegicus | ADP + phosphorylated neuronal nitric-oxide synthase | - |
? |
Synonyms | Comment | Organism |
---|---|---|
calcium/calmodulin-dependent protein kinase I | - |
Rattus norvegicus |
calcium/calmodulin-dependent protein kinase II | - |
Rattus norvegicus |
CaM-dependent protein kinase I | - |
Rattus norvegicus |
CaM-dependent protein kinase II | - |
Rattus norvegicus |
CaM-K Ialpha | - |
Rattus norvegicus |
CaM-K IIalpha | - |
Rattus norvegicus |
CaM-K IV | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |