Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | determination of the calmodulin binding regulatory domain | Physarum polycephalum |
Cloned (Comment) | Organism |
---|---|
cNA library construction and screening, DNA and amino acid sequence determination and analsis, expression in Escherichia coli strain BL21(DE3) with or without His-tag | Physarum polycephalum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Physarum polycephalum | |
Mg2+ | - |
Physarum polycephalum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42519 | - |
x * 42519, amino acid sequence calculation | Physarum polycephalum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physarum polycephalum | Q8WSQ3 | - |
- |
Physarum polycephalum Ng-1 | Q8WSQ3 | - |
- |
Purification (Comment) | Organism |
---|---|
non-tagged recombinant enzyme from Escherichia coli by CaM affinity chromatography and gel filtration, or His-tagged recombinant enzyme by nickel affinity chromatography | Physarum polycephalum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
plasmodium | - |
Physarum polycephalum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + myosin II light chain | recombinant substrate from Physarum polycephalum, phosphorylation at Ser18, phosphorylation site analysis, no phosphorylation of substrate mutant S18A | Physarum polycephalum | ADP + phosphorylated myosin II light chain | - |
? | |
ATP + myosin II light chain | recombinant substrate from Physarum polycephalum, phosphorylation at Ser18, phosphorylation site analysis, no phosphorylation of substrate mutant S18A | Physarum polycephalum Ng-1 | ADP + phosphorylated myosin II light chain | - |
? | |
additional information | no activity with casein, bovine serum albumin, and light chains of various myosins from Dictyostelium, scallop, Mytilis, and chicken | Physarum polycephalum | ? | - |
? | |
additional information | no activity with casein, bovine serum albumin, and light chains of various myosins from Dictyostelium, scallop, Mytilis, and chicken | Physarum polycephalum Ng-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42519, amino acid sequence calculation | Physarum polycephalum |
Synonyms | Comment | Organism |
---|---|---|
calmodulin-dependent protein kinase | - |
Physarum polycephalum |
CaM-dependent protein kinase | - |
Physarum polycephalum |
CaMK | - |
Physarum polycephalum |
More | the enzyme belongs to the family of calmodulin-dependent kinases, cf. EC 2.7.11.18 | Physarum polycephalum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at | Physarum polycephalum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Physarum polycephalum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Physarum polycephalum |