Cloned (Comment) | Organism |
---|---|
expression of GST-tagged GRK5 in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
RNA aptamer C13 | development and synthesis of a highly specific RNA aptamer C13 that potently inhibits GRK2, EC 2.7.11.15, and GRK5, inhibitor secondary structure, overview | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34947 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged GRK5 from insect Sf9 cells by ion exchange and heparin affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | GRK5 also phosphorylates rhodopsin, cf. EC 2.7.11.14 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G-protein-coupled receptor kinase 5 | - |
Homo sapiens |
GRK5 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000079 | - |
inhibition of GRK5 | Homo sapiens | RNA aptamer C13 |