Cloned (Comment) | Organism |
---|---|
transient overexpression of wild-types and dominant negative mutants of GRK5 and GRK6 in COS-7 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K215R | inactive dominant negative mutants of GRK5 or GRK6, overexpression of the GRK5 mutant in myometrial cells does not influence beta-adrenergic receptor sensitivity, while overexpression of GRK6 mutant leads to a 70% increase in isoproterenol-stimulated beta-adrenergic signaling via the beta-adrenergic receptor | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | GRK inhibitor | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | the GRK4-6 family member enzymes mediate beta-adrenergic receptor desensitization, the beta-adrenergic receptor is stimulated by agonists such as isoproterenol, cholera toxin, or forskolin, and induces cAMP production | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
myometrium | primary cells | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | urea treated outer segments of rhodopsin | Rattus norvegicus | ADP + phosphorylated rhodopsin | - |
? | |
additional information | the GRK4-6 family member enzymes mediate beta-adrenergic receptor desensitization, the beta-adrenergic receptor is stimulated by agonists such as isoproterenol, cholera toxin, or forskolin, and induces cAMP production | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase 5 | - |
Rattus norvegicus |
G protein-coupled receptor kinase 6 | - |
Rattus norvegicus |
GRK5 | - |
Rattus norvegicus |
GRK6 | - |
Rattus norvegicus |
More | the enzyme belongs to the GRK4-6 family | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |