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Literature summary for 2.7.11.15 extracted from

  • Sherrill, J.D.; Miller, W.E.
    G protein-coupled receptor (GPCR) kinase 2 regulates agonist-independent Gq/11 signaling from the mouse cytomegalovirus GPCR M33 (2006), J. Biol. Chem., 281, 39796-39805.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression of Myc-tagged wild-type and mutant GRK2s in HEK-293T cells, co-expression with M33 in wild-type and Galphaq/11-/- mouse embryonic fibroblasts, M33 couples directly to the Gq/11 signaling pathway to induce high levels of total inositol phosphates in an agonist-independent manner Mus musculus

Protein Variants

Protein Variants Comment Organism
D110A the point mutation within the RH domain abrogates GRK2 sequestration of activated GTP-boundGalphaq/11 Mus musculus
D110A/K220R the mutant, which exhibits no RH or kinase activity, is completely defective in its ability to attenuate M33 signaling Mus musculus
K220R the point mutation within the kinase domain inhibits GRK2 catalytic activity Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + protein M33 Mus musculus GRK2 is a potent regulator of the mouse cytomegalovirus GPCR protein M33-induced Gq/11 signaling through its ability to phosphorylate M33 and sequester Galphaq/11 proteins dependent on an intact RH domain, the protein M33 is able to induce inositol phosphate accumulation, activate NF-kappaB, and promote smooth muscle cell migration, viral GPCRs like M33 play a role in viral dissemination in vivo, M33 is required for efficient murine cytomegalovirus replication in the mouse, and induces several signlaing pathways, overview ADP + phosphorylated protein M33
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 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + protein M33 GRK2 is a potent regulator of the mouse cytomegalovirus GPCR protein M33-induced Gq/11 signaling through its ability to phosphorylate M33 and sequester Galphaq/11 proteins dependent on an intact RH domain, the protein M33 is able to induce inositol phosphate accumulation, activate NF-kappaB, and promote smooth muscle cell migration, viral GPCRs like M33 play a role in viral dissemination in vivo, M33 is required for efficient murine cytomegalovirus replication in the mouse, and induces several signlaing pathways, overview Mus musculus ADP + phosphorylated protein M33
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 ?
ATP + protein M33 recombinant FLAG-tagged M33 expressed in HEK-293T cells Mus musculus ADP + phosphorylated protein M33
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 ?

Synonyms

Synonyms Comment Organism
G protein-coupled receptor kinase 2
-
 Mus musculus
GPCR kinase 2
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus