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Literature summary for 2.7.11.15 extracted from
- Specificity of G protein-coupled receptor kinase 6-mediated phosphorylation and regulation of single-cell M3 muscarinic acetylcholine receptor signaling (2003), Mol. Pharmacol., 64, 1059-1068.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| stable overexpression of GFP-fusion K220R mutant GRK2 and GFP-fusion K220R mutant GRK3 in SH-SY5Y neuroblastoma cells via adenovirus infection system | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K220R | site-directed mutagenesis, inactive, dominant negative mutants of GRK3 and GRK2, overexpression in SH-SY5Y cells does not influence endogenous M3 muscarinic acetylcholine receptor phosphorylation and desensitization by the endogenous wild-type GRK6, but inhibits methacholine-stimulated inositol 3-phosphate production by 75% | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | GRK2 and GRK3 are involved in methacholine-stimulated inositol 3-phosphate production | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| SH-SY5Y cell | neuroblastoma cell line | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | GRK2 and GRK3 are involved in methacholine-stimulated inositol 3-phosphate production | Homo sapiens | ? | - |
? | |
| additional information | the M3 muscarinic acetylcholine receptor is no substrate for GRK2 and GRK3 | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G protein-coupled receptor kinase 2 | - |
Homo sapiens |
| G protein-coupled receptor kinase 3 | - |
Homo sapiens |
| GRK2 | - |
Homo sapiens |
| GRK3 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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