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Literature summary for 2.7.11.15 extracted from

  • Yoshida, N.; Haga, K.; Haga, T.
    Identification of sites of phosphorylation by G-protein-coupled receptor kinase 2 in beta-tubulin (2003), Eur. J. Biochem., 270, 1154-1163.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GRK2 in Spodoptera frugiperda Sf9 cells Homo sapiens

General Stability

General Stability Organism
glycerol stabilizes Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
microtubule
-
Homo sapiens 5874
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GRK2 from insect Sf9 cells by ammonium sulfate fractionation, hydrophobic interaction and heparin affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + dephospho-beta-tubulin purified substrate from porcine brain or human GST-tagged protein recombinantly expressed in Escherichia coli, phosphorylation of betaI- and betaIII-tubulin at Thr409, Ser420, and, in betaIII-tubulin, also at Ser420 of the C-terminal outer surface of the substrate protein Homo sapiens ADP + phosphorylated beta-tubulin
-
?
ATP + phosducin
-
Homo sapiens ADP + phosphorylated phosducin
-
?
ATP + synuclein
-
Homo sapiens ADP + phosphorylated synuclein
-
?
additional information GRK2 prefers acidic protein sequences for phosphorylation Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
G-protein-coupled receptor kinase 2
-
Homo sapiens
GRK2
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens