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Literature summary for 2.7.11.15 extracted from
- Purification and functional characterization of beta-adrenergic receptor kinase expressed in insect cells (1993), FEBS Lett., 324, 59-62.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system | Homo sapiens |
| beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0038 | - |
rhodopsin | pH 7.5, 30°C, recombinant beta-ARK 1 | Bos taurus |  |
| 0.022 | - |
ATP | pH 7.5, 30°C, recombinant beta-ARK 1, rhodopsin as substrate | Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
| cytosol | - |
Bos taurus | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Homo sapiens | |
| Mg2+ | requirement | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-adrenergic receptor | Homo sapiens | natural substrate: beta2-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Bos taurus | natural substrate: beta2-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Homo sapiens | agonist-occupied form of the receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Bos taurus | agonist-occupied form of the receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Homo sapiens | involved in homologous desensitization of beta-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Bos taurus | involved in homologous desensitization of beta-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
enzyme exists in 2 subtypes: beta-ARK 1 and beta-ARK 2 | - |
| Bos taurus | - |
major isoform beta-ARK 1 | - |
| Homo sapiens | - |
major isoform beta-ARK 1 | - |
| Homo sapiens | - |
enzyme exists in 2 subtypes: beta-ARK1 and 2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant beta-ARK 1 expressed in Sf9 cells | Bos taurus |
| recombinant beta-ARK 1, expressed in Sf9 cells | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-adrenergic receptor | specifically phosphorylates the agonist-occupied form of the receptor | Homo sapiens | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | specifically phosphorylates the agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | human beta2-adrenergic receptor | Homo sapiens | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | human beta2-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | incorporation of up to 5 mol phosphate/mol receptor | Homo sapiens | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | incorporation of up to 5 mol phosphate/mol receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | beta-AR from Sf9 cells | Homo sapiens | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | beta-AR from Sf9 cells | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | natural substrate: beta2-adrenergic receptor | Homo sapiens | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | natural substrate: beta2-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | agonist-occupied form of the receptor | Homo sapiens | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | involved in homologous desensitization of beta-adrenergic receptor | Homo sapiens | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | involved in homologous desensitization of beta-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + rhodopsin | in form of bovine rod outer segments | Homo sapiens | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | in form of bovine rod outer segments | Bos taurus | ADP + phosphorhodopsin | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
| ATP | cofactor ATP | Homo sapiens | |
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