Cloned (Comment) | Organism |
---|---|
expression of His6-tagged wild-type and mutant enzymes in High Five insect cells using the baculovirus transfection system | Bos taurus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, X-ray diffraction structure determination and analysis, molecular replacement | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
D164A | site-directed mutagenesis, a dimer interface mutant showing only slightly altered kinetics compared to the wild-type enzyme | Bos taurus |
D164A/L166K | site-directed mutagenesis, a dimer interface mutant showing reduced activity compared to the wild-type enzyme | Bos taurus |
D164A/W531A | site-directed mutagenesis, inactive mutant | Bos taurus |
L166K | site-directed mutagenesis, a dimer interface mutant showing reduced activity compared to the wild-type enzyme | Bos taurus |
L166K/W531A | site-directed mutagenesis, inactive mutant | Bos taurus |
S5A | site-directed mutagenesis, an N-terminal mutant showing reduced activity compared to the wild-type enzyme | Bos taurus |
S5D | site-directed mutagenesis, inactive mutant | Bos taurus |
T8A | site-directed mutagenesis, an N-terminal mutant showing reduced activity compared to the wild-type enzyme | Bos taurus |
T8D | site-directed mutagenesis, inactive mutant | Bos taurus |
T8E | site-directed mutagenesis, an N-terminal mutant showing reduced activity compared to the wild-type enzyme | Bos taurus |
W531A | site-directed mutagenesis, inactive mutant | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Bos taurus | |
0.0015 | - |
rhodopsin | pH 7.5, 30°C, mutant T8A | Bos taurus | |
0.0021 | - |
rhodopsin | pH 7.5, 30°C, wild-type enzyme | Bos taurus | |
0.0031 | - |
rhodopsin | pH 7.5, 30°C, mutant D164A/L166K | Bos taurus | |
0.0038 | - |
rhodopsin | pH 7.5, 30°C, mutant D164A | Bos taurus | |
0.0048 | - |
rhodopsin | pH 7.5, 30°C, mutant T8E | Bos taurus | |
0.0051 | - |
rhodopsin | pH 7.5, 30°C, mutant S5A | Bos taurus | |
0.0067 | - |
rhodopsin | pH 7.5, 30°C, mutant L166K | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | Bos taurus | - |
ADP + phosphorhodopsin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P28327 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from High Five insect cells by nickel affinity chromatography and ion exchange chromatography | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + rhodopsin | - |
Bos taurus | ADP + phosphorhodopsin | - |
? | |
additional information | nucleotide binding and conformational change, overview | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | domain structure analysis, mass spectrometric analysis, overview | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
GRK1 | - |
Bos taurus |
More | GRK1 is the founding member of the G protein-coupled receptor kinase, GRK kinase, family | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |